Document Detail


Subclass specificity of autoantibodies against myosin in patients with idiopathic dilated cardiomyopathy: pro-inflammatory antibodies in DCM patients.
MedLine Citation:
PMID:  10362496     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Detection of antimyosin antibodies in non-inflammatory cardiac disease undermines their disease specificity as a sensitive marker of damage in dilated cardiomyopathy (DCM) patients. Antibody subclass specificity could provide a more sensitive marker of disease and possibly discriminate the humoral autoimmune responses in different cardiac diseases. Frequency and reactivity of autoantibodies against alpha- and beta-isoforms of myosin heavy chain (mhc) were evaluated by ELISA for IgG, IgM, and subclasses IgG1, IgG2, and IgG3 in patients with DCM (NYHA III/IV, n = 82), end stage ischemic heart disease (E-IHD: NYHA III/IV, n = 62), mild ischemic heart disease (NYHA I/II, n = 27), and controls (n = 54). Autoantibodies against atrial and ventricular myosin were raised in heart failure patients compared to mild-IHD and controls but with different antigen affinities. Reactivity in E-IHD was significantly raised against (ventricular) beta-mhc compared with only mild-IHD patients, suggesting a relative increase in ventricular specific antibodies in IHD patients with a higher NYHA class. IgG subclass analysis for IgG1, IgG2, and IgG3 against alpha- and beta-mhc showed statistically raised levels of IgG3 only in DCM patients and a significantly higher reactivity of IgG2 in heart failure patients versus controls. The results demonstrate immunological heterogeneity of antimyosin antibodies developed in different clinical entities. Pro-inflammatory characteristics of IgG3 antibodies in a select group of patients with DCM may contribute to autoimmune mechanisms of injury in these patients.
Authors:
R S Warraich; M J Dunn; M H Yacoub
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  259     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1999 Jun 
Date Detail:
Created Date:  1999-06-28     Completed Date:  1999-06-28     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  255-61     Citation Subset:  IM    
Copyright Information:
Copyright 1999 Academic Press.
Affiliation:
National Heart and Lung Institute, Harefield Hospital, Harefield, Middlesex, UB9 6JH, United Kingdom.
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MeSH Terms
Descriptor/Qualifier:
Autoantibodies / classification,  immunology*
Cardiomyopathy, Dilated / immunology*
Female
Humans
Immunoglobulin G / classification,  immunology
Immunoglobulin M / immunology
Immunoglobulins / classification,  immunology
Inflammation / immunology
Male
Middle Aged
Myosin Heavy Chains / immunology
Myosins / immunology*
Protein Isoforms / immunology
Chemical
Reg. No./Substance:
0/Autoantibodies; 0/Immunoglobulin G; 0/Immunoglobulin M; 0/Immunoglobulins; 0/Myosin Heavy Chains; 0/Protein Isoforms; EC 3.6.4.1/Myosins

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