Document Detail

Subcellular redistribution of the renal betaine transporter during hypertonic stress.
MedLine Citation:
PMID:  12839828     Owner:  NLM     Status:  MEDLINE    
The betaine transporter (BGT1) protects cells in the hypertonic renal inner medulla by mediating uptake and accumulation of the osmolyte betaine. Transcriptional regulation plays an essential role in upregulation of BGT1 transport when renal cells are exposed to hypertonic medium for 24 h. Posttranscriptional regulation of the BGT1 protein is largely unexplored. We have investigated the distribution of BGT1 protein in live cells after transfection with BGT1 tagged with enhanced green fluorescent protein (EGFP). Fusion of EGFP to the NH2 terminus of BGT1 produced a fusion protein (EGFP-BGT) with transport properties identical to normal BGT1, as determined by ion dependence, inhibitor sensitivity, and apparent Km for GABA. Confocal microscopy of EGFP-BGT fluorescence in transfected Madin-Darby canine kidney (MDCK) cells showed that hypertonic stress for 24 h induced a shift in subcellular distribution from cytoplasm to plasma membrane. This was confirmed by colocalization with anti-BGT1 antibody staining. In fibroblasts, transfected EGFP-BGT caused increased transport in response to hypertonic stress. The activation of transport was not accompanied by increased expression of EGFP-BGT, as determined by Western blotting. Membrane insertion of EGFP-BGT protein in MDCK cells began within 2-3 h after onset of hypertonic stress and was blocked by cycloheximide. We conclude that posttranscriptional regulation of BGT1 is essential for adaptation to hypertonic stress and that insertion of BGT1 protein to the plasma membrane may require accessory proteins.
Stephen A Kempson; Vaibhave Parikh; Lixuan Xi; Shaoyou Chu; Marshall H Montrose
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.     Date:  2003-07-02
Journal Detail:
Title:  American journal of physiology. Cell physiology     Volume:  285     ISSN:  0363-6143     ISO Abbreviation:  Am. J. Physiol., Cell Physiol.     Publication Date:  2003 Nov 
Date Detail:
Created Date:  2003-10-08     Completed Date:  2003-12-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100901225     Medline TA:  Am J Physiol Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  C1091-100     Citation Subset:  IM    
Department of Cellular and Integrative Medicine, Indiana University School of Medicine, Medical Sciences 451, 635 Barnhill Drive, Indianapolis, IN 46202-5120, USA.
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MeSH Terms
Carrier Proteins / metabolism*
Cell Line
Cell Membrane / drug effects,  metabolism*
Cells, Cultured
Hypertonic Solutions
Kidney / drug effects,  metabolism*,  ultrastructure*
Mice, Inbred C3H
Protein Transport / drug effects,  physiology
Sucrose / pharmacology
gamma-Aminobutyric Acid / metabolism
Reg. No./Substance:
0/Carrier Proteins; 0/Hypertonic Solutions; 146313-33-9/betaine plasma membrane transport proteins; 56-12-2/gamma-Aminobutyric Acid; 57-50-1/Sucrose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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