Document Detail

Subcellular phototoxicity of 5-aminolaevulinic acid (ALA).
MedLine Citation:
PMID:  9443145     Owner:  NLM     Status:  MEDLINE    
BACKGROUND AND OBJECTIVE: 5-aminolaevulinic acid (ALA) is a new, promising photosensitizer for PDT of cancer. Subcellular toxicity induced by ALA and light exposure in single cells was studied to elucidate the mechanism of cell damage. STUDY DESIGN/MATERIALS AND METHODS: CPAE, PTK2, and rat neonatal myocardial cells treated with ALA were examined for localization using fluorescence microscopy and for subcellular phototoxicity using 630 nm laser microbeam irradiation of specific subcellular regions. RESULTS: In CPAE and PTK2 cells, a large amount of fluorescence was detected in the peri-nuclear cytoplasm. In rat neonatal myocardial cells, the sensitizer selectively localized in the large mitochondria. In both cell types, there was little phototoxicity when the peripheral cytoplasmic region was exposed, as compared to considerable phototoxicity with exposure of either the perinuclear or nuclear regions. CONCLUSION: Both the CPAE and PTK2 cells demonstrated that the nucleus followed by the perinuclear cytoplasm are the most sensitive cell areas with no sensitivity in the peripheral cytoplasm.
H Liang; D S Shin; Y E Lee; D C Nguyen; T C Trang; A H Pan; S L Huang; D H Chong; M W Berns
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Lasers in surgery and medicine     Volume:  22     ISSN:  0196-8092     ISO Abbreviation:  Lasers Surg Med     Publication Date:  1998  
Date Detail:
Created Date:  1998-03-09     Completed Date:  1998-03-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8007168     Medline TA:  Lasers Surg Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  14-24     Citation Subset:  IM    
Beckman Laser Institute and Medical Clinic, University of California, Irvine 92612, USA.
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MeSH Terms
Aminolevulinic Acid / toxicity*
Cell Nucleus / drug effects
Cells, Cultured
Cytoplasm / drug effects
Endothelium, Vascular / cytology,  drug effects*
Heart / drug effects*
Kidney / cytology,  drug effects*
Microscopy, Fluorescence
Mitochondria / drug effects
Myocardium / cytology
Photosensitizing Agents / toxicity*
Grant Support
Reg. No./Substance:
0/Photosensitizing Agents; 106-60-5/Aminolevulinic Acid

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