Document Detail


Subcellular phototoxicity of 5-aminolaevulinic acid (ALA).
MedLine Citation:
PMID:  9443145     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND AND OBJECTIVE: 5-aminolaevulinic acid (ALA) is a new, promising photosensitizer for PDT of cancer. Subcellular toxicity induced by ALA and light exposure in single cells was studied to elucidate the mechanism of cell damage. STUDY DESIGN/MATERIALS AND METHODS: CPAE, PTK2, and rat neonatal myocardial cells treated with ALA were examined for localization using fluorescence microscopy and for subcellular phototoxicity using 630 nm laser microbeam irradiation of specific subcellular regions. RESULTS: In CPAE and PTK2 cells, a large amount of fluorescence was detected in the peri-nuclear cytoplasm. In rat neonatal myocardial cells, the sensitizer selectively localized in the large mitochondria. In both cell types, there was little phototoxicity when the peripheral cytoplasmic region was exposed, as compared to considerable phototoxicity with exposure of either the perinuclear or nuclear regions. CONCLUSION: Both the CPAE and PTK2 cells demonstrated that the nucleus followed by the perinuclear cytoplasm are the most sensitive cell areas with no sensitivity in the peripheral cytoplasm.
Authors:
H Liang; D S Shin; Y E Lee; D C Nguyen; T C Trang; A H Pan; S L Huang; D H Chong; M W Berns
Related Documents :
9496685 - Generation of dendritic cells from peripheral blood adherent cells in medium with human...
8051175 - Activation of src-like p56/p53lyn tyrosine kinase by ionizing radiation.
9191895 - Adenine-induced arrest of mammalian cells in early s-phase is related to the prevention...
20398405 - Damaged dna-binding protein 2 (ddb2) protects against uv irradiation in human cells and...
3556285 - Presence of pancreatic hormones in islet cells with mhc-class ii antigen expression.
9689115 - Lectin-like oxidized low-density lipoprotein receptor 1 mediates phagocytosis of aged/a...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Lasers in surgery and medicine     Volume:  22     ISSN:  0196-8092     ISO Abbreviation:  Lasers Surg Med     Publication Date:  1998  
Date Detail:
Created Date:  1998-03-09     Completed Date:  1998-03-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8007168     Medline TA:  Lasers Surg Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  14-24     Citation Subset:  IM    
Affiliation:
Beckman Laser Institute and Medical Clinic, University of California, Irvine 92612, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Aminolevulinic Acid / toxicity*
Animals
Cattle
Cell Nucleus / drug effects
Cells, Cultured
Cytoplasm / drug effects
Dipodomys
Endothelium, Vascular / cytology,  drug effects*
Female
Heart / drug effects*
Kidney / cytology,  drug effects*
Lasers
Microscopy, Fluorescence
Mitochondria / drug effects
Myocardium / cytology
Photochemotherapy*
Photosensitizing Agents / toxicity*
Rats
Grant Support
ID/Acronym/Agency:
R01CA32248/CA/NCI NIH HHS; RR01192/RR/NCRR NIH HHS
Chemical
Reg. No./Substance:
0/Photosensitizing Agents; 106-60-5/Aminolevulinic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Treatment of cutaneous hemangiomas in preterm neonatal twins with the flashlamp-pumped pulsed dye la...
Next Document:  Clinical preconditions and treatment modality: effects on pulp surgery outcome.