Document Detail


Subcellular localization of the mosquito sterol carrier protein-2 and sterol carrier protein-x.
MedLine Citation:
PMID:  15145982     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Subcellular distribution of Aedes aegypti sterol carrier protein-2 (AeSCP-2) and AeSCP-x was studied using electron microscopy. In both cultured A. aegypti cells and in the larval midgut, AeSCP-2 was detected mostly in the cytosol, with some labeling mitochondria and nucleus, but not in membranous vesicles. The widespread distribution of AeSCP-2 in the midgut epithelium is consistent with its potential lipid transfer function in all phases of cholesterol absorption. In contrast, AeSCP-x was found mostly in the peroxisome. Differences in the subcellular distribution of AeSCP-2 and AeSCP-x suggest that these two members of the SCP-2 gene family are functionally distinct. Overexpression of AeSCP-2 in A. aegypti cells showed increased localization of AeSCP-2 to cytosol, mitochondria, and nucleus. This is the first report on the nuclear distribution of an SCP. Overexpression of AeSCP-2 resulted in increased cholesterol incorporation in cells, suggesting that AeSCP-2 enhances cholesterol uptake.
Authors:
Que Lan; Randall J Massey
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2004-05-16
Journal Detail:
Title:  Journal of lipid research     Volume:  45     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  2004 Aug 
Date Detail:
Created Date:  2004-07-15     Completed Date:  2005-02-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1468-74     Citation Subset:  IM    
Copyright Information:
Copyright 2004 American Society for Biochemistry and Molecular Biology, Inc.
Affiliation:
Department of Entomology, University of Wisconsin-Madison, Madison, WI 53706, USA. qlan@entomology.wisc.edu
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MeSH Terms
Descriptor/Qualifier:
Aedes / immunology,  metabolism*
Animals
Antibodies / immunology
Carrier Proteins / immunology,  metabolism*
Gastrointestinal Tract / metabolism
Larva / metabolism
Microscopy, Immunoelectron
Chemical
Reg. No./Substance:
0/Antibodies; 0/Carrier Proteins; 0/sterol carrier proteins

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