Document Detail

Subcellular localization and characterization of G protein-coupled receptor homolog from lymphocystis disease virus isolated in China.
MedLine Citation:
PMID:  17425429     Owner:  NLM     Status:  MEDLINE    
G protein-coupled receptors (GPCRs) constitute a large superfamily involved in various types of signal transduction pathways, and play an important role in coordinating the activation and migration of leukocytes to sites of infection and inflammation. Viral GPCRs, on the other hand, can help the virus to escape from host immune surveillance and contribute to viral pathogenesis. Lymphocystis disease virus isolated in China (LCDV-C) contains a putative homolog of cellular GPCRs, LCDV-C GPCR. In this paper, LCDV-C GPCR was cloned, and the subcellular localization and characterization of GPCR protein were investigated in fish cells. LCDV-C GPCR encoded a 325 amino acid peptide, containing a typical seven-transmembrane domain characteristic of the chemokine receptors and a conserved DRY motif that is usually essential for receptor activation. Transient transfection of GPCR-EGFP in fathead minnow (FHM) cells and epithelioma papulosum cyprini (EPC) cells indicated that LCDV-C GPCR was expressed abundantly in both the cytoplasm and nucleoplasm. Transient overexpression of GPCR in these two cells cannot induce obvious apoptosis. FHM cells stably expressing GPCR showed enhanced cell proliferation and significant anchorage-independent growth. The effects of GPCR protein on external apoptotic stimuli were examined. Few apoptotic bodies were observed in cells expressing GPCR treated with actinomycin D (ActD). Quantitative analysis of apoptotic cells indicated that a considerable decrease in the apoptotic fraction of cells expressing GPCR, compared with the control cells, was detected after exposure to ActD and cycloheximide. These data suggest that LCDV-C GPCR may inhibit apoptosis as part of its potential mechanism in mediating cellular transformation.
Youhua Huang; Xiaohong Huang; Jing Zhang; Jianfang Gui; Qiya Zhang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Viral immunology     Volume:  20     ISSN:  0882-8245     ISO Abbreviation:  Viral Immunol.     Publication Date:  2007  
Date Detail:
Created Date:  2007-04-11     Completed Date:  2007-05-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8801552     Medline TA:  Viral Immunol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  150-9     Citation Subset:  IM    
State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Graduate University of Chinese Academy of Science, Wuhan, People's Republic of China.
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MeSH Terms
Amino Acid Sequence
Cell Proliferation
Cells, Cultured
Fishes / virology*
Iridoviridae / chemistry*
Molecular Sequence Data
Receptors, G-Protein-Coupled / analysis*,  chemistry,  physiology
Reg. No./Substance:
0/Receptors, G-Protein-Coupled

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