Document Detail


Subcellular localization of beta-catenin in malignant cell lines and squamous cell carcinomas of the oral cavity.
MedLine Citation:
PMID:  12165056     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Beta-catenin, an E-cadherin-associated protein involved in cell-cell adhesion and signaling, has been hypothesized to translocate to the nucleus and activate transcription in several human cancers, including oral squamous cell carcinomas (OSCC). METHODS: In the present study, we analyzed the subcellular localization of beta-catenin in cultures of human oral normal and malignant (cell lines SCC15 and SCC25) keratinocytes and in 24 frozen samples of oral squamous cell carcinomas by a double-staining technique for nucleic acids and beta-catenin. Growth potential, as assessed by cell count at different time periods, was established for normal, SCC15 and SCC25 cell lines; oral squamous cell carcinomas were classified according to the histopathological and malignancy indexes. RESULTS: Beta-catenin localized at the plasma membrane in the normal and SCC15 cells, not in the SCC25 cells, where it localized mostly in the perinuclear and nuclear areas. In the growth assays, SCC25 cell lines proliferated faster than in normal and SCC15 cells over a period of 6 days (cell numbers were significantly different, P < 0.0001). Carcinoma sections showed a combination of membranous, cytoplasmic and, in few invading epithelial islands of two tumors, nuclear localization of beta-catenin. CONCLUSIONS: In oral squamous cell carcinomas, nuclear beta-catenin staining was observed only within invading islands of two carcinomas deep in the underlying connective tissue. On the basis of this study, we conclude that intranuclear beta-catenin does not appear to be a common finding in oral squamous cell carcinomas and that a clear association between intranuclear beta-catenin and histopathological and malignancy indexes in vivo could not be established.
Authors:
Alberto Gasparoni; A Chaves; L Fonzi; G K Johnson; G B Schneider; C A Squier
Related Documents :
1544706 - Immunohistochemical evaluation of parathyroid hormone-related protein (pthrp) in the ut...
7556886 - Parathyroid hormone-related peptide and 8701-bc breast cancer cell growth and invasion ...
2445466 - Monoclonal antibodies against human oral squamous cell carcinoma reacting with keratin ...
6846426 - Basal cell pseudopodia and the degree of maturation of squamous metaplasia of the uteri...
21519146 - Mommy - where do tumors come from?
25367756 - Cover picture.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology     Volume:  31     ISSN:  0904-2512     ISO Abbreviation:  J. Oral Pathol. Med.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-08-07     Completed Date:  2002-12-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8911934     Medline TA:  J Oral Pathol Med     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  385-94     Citation Subset:  D; IM    
Affiliation:
Dows Institute for Dental Research, College of Dentistry, University of Iowa, IA 52242, USA. alberto-gasparoni@uiowa.edu
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Cadherins / analysis*
Carcinoma, Squamous Cell / ultrastructure*
Cell Count
Cell Culture Techniques
Cell Division
Cell Membrane / ultrastructure
Cell Nucleus / ultrastructure
Child
Connective Tissue / ultrastructure
Cytoskeletal Proteins / analysis*
Epithelial Cells / ultrastructure
Gingiva / ultrastructure
Humans
Keratinocytes / ultrastructure
Microscopy, Confocal
Middle Aged
Mouth Mucosa / ultrastructure
Mouth Neoplasms / ultrastructure*
Subcellular Fractions / ultrastructure*
Trans-Activators / analysis*
Tumor Cells, Cultured
beta Catenin
Chemical
Reg. No./Substance:
0/CTNNB1 protein, human; 0/Cadherins; 0/Cytoskeletal Proteins; 0/Trans-Activators; 0/beta Catenin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Cyclosporine A protects against apoptosis in ischaemic/reperfused rat kidneys.
Next Document:  Mutations of the adenomatous polyposis coli gene in areca quid and tobacco-associated oral squamous ...