Document Detail

Subcellular localization of beta-catenin in malignant cell lines and squamous cell carcinomas of the oral cavity.
MedLine Citation:
PMID:  12165056     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Beta-catenin, an E-cadherin-associated protein involved in cell-cell adhesion and signaling, has been hypothesized to translocate to the nucleus and activate transcription in several human cancers, including oral squamous cell carcinomas (OSCC). METHODS: In the present study, we analyzed the subcellular localization of beta-catenin in cultures of human oral normal and malignant (cell lines SCC15 and SCC25) keratinocytes and in 24 frozen samples of oral squamous cell carcinomas by a double-staining technique for nucleic acids and beta-catenin. Growth potential, as assessed by cell count at different time periods, was established for normal, SCC15 and SCC25 cell lines; oral squamous cell carcinomas were classified according to the histopathological and malignancy indexes. RESULTS: Beta-catenin localized at the plasma membrane in the normal and SCC15 cells, not in the SCC25 cells, where it localized mostly in the perinuclear and nuclear areas. In the growth assays, SCC25 cell lines proliferated faster than in normal and SCC15 cells over a period of 6 days (cell numbers were significantly different, P < 0.0001). Carcinoma sections showed a combination of membranous, cytoplasmic and, in few invading epithelial islands of two tumors, nuclear localization of beta-catenin. CONCLUSIONS: In oral squamous cell carcinomas, nuclear beta-catenin staining was observed only within invading islands of two carcinomas deep in the underlying connective tissue. On the basis of this study, we conclude that intranuclear beta-catenin does not appear to be a common finding in oral squamous cell carcinomas and that a clear association between intranuclear beta-catenin and histopathological and malignancy indexes in vivo could not be established.
Alberto Gasparoni; A Chaves; L Fonzi; G K Johnson; G B Schneider; C A Squier
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology     Volume:  31     ISSN:  0904-2512     ISO Abbreviation:  J. Oral Pathol. Med.     Publication Date:  2002 Aug 
Date Detail:
Created Date:  2002-08-07     Completed Date:  2002-12-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8911934     Medline TA:  J Oral Pathol Med     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  385-94     Citation Subset:  D; IM    
Dows Institute for Dental Research, College of Dentistry, University of Iowa, IA 52242, USA.
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MeSH Terms
Cadherins / analysis*
Carcinoma, Squamous Cell / ultrastructure*
Cell Count
Cell Culture Techniques
Cell Division
Cell Membrane / ultrastructure
Cell Nucleus / ultrastructure
Connective Tissue / ultrastructure
Cytoskeletal Proteins / analysis*
Epithelial Cells / ultrastructure
Gingiva / ultrastructure
Keratinocytes / ultrastructure
Microscopy, Confocal
Middle Aged
Mouth Mucosa / ultrastructure
Mouth Neoplasms / ultrastructure*
Subcellular Fractions / ultrastructure*
Trans-Activators / analysis*
Tumor Cells, Cultured
beta Catenin
Reg. No./Substance:
0/CTNNB1 protein, human; 0/Cadherins; 0/Cytoskeletal Proteins; 0/Trans-Activators; 0/beta Catenin

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