Document Detail

Subcellular localization analysis of the closely related Fps/Fes and Fer protein-tyrosine kinases suggests a distinct role for Fps/Fes in vesicular trafficking.
MedLine Citation:
PMID:  11339827     Owner:  NLM     Status:  MEDLINE    
The subcellular localizations of the Fps/Fes and closely related Fer cytoplasmic tyrosine kinases were studied using green fluorescent protein (GFP) fusions and confocal fluorescence microscopy. In contrast to previous reports, neither kinase localized to the nucleus. Fer was diffusely cytoplasmic throughout the cell cycle. Fps/Fes also displayed a diffuse cytoplasmic localization, but in addition it showed distinct accumulations in cytoplasmic vesicles as well as in a perinuclear region consistent with the Golgi. This localization was very similar to that of TGN38, a known marker of the trans Golgi. The localization of Fps/Fes and TGN38 were both perturbed by brefeldin A, a fungal metabolite that disrupts the Golgi apparatus. Fps/Fes was also found to colocalize to various extents with several Rab proteins, which are members of the monomeric G-protein superfamily involved in vesicular transport between specific subcellular compartments. Using Rabs that are involved in endocytosis (Rab5B and Rab7) or exocytosis (Rab1A and Rab3A), we showed that Fps/Fes is localized in both pathways. These results suggest that Fps/Fes may play a general role in the regulation of vesicular trafficking.
R Zirngibl; D Schulze; S E Mirski; S P Cole; P A Greer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental cell research     Volume:  266     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-05-07     Completed Date:  2001-06-14     Revised Date:  2012-06-01    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  87-94     Citation Subset:  IM    
Copyright Information:
Copyright 2001 Academic Press.
Cancer Research Laboratories, Queen's University, Kingston, Ontario K7L 3N6, Canada.
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MeSH Terms
COS Cells / cytology,  metabolism
Cell Compartmentation / physiology*
Cell Cycle / physiology
Cytoplasm / metabolism*,  ultrastructure
Fusion Proteins, gag-onc / metabolism*
Golgi Apparatus / metabolism
Intracellular Membranes / metabolism
Membrane Glycoproteins / metabolism
Membrane Proteins*
Protein Transport / physiology*
Protein-Tyrosine Kinases*
Proto-Oncogene Proteins / metabolism*
Transport Vesicles / metabolism*,  ultrastructure
rab GTP-Binding Proteins / metabolism
Reg. No./Substance:
0/Fusion Proteins, gag-onc; 0/Glycoproteins; 0/Membrane Glycoproteins; 0/Membrane Proteins; 0/Proto-Oncogene Proteins; 110736-90-8/proto-oncogene protein c-fes-fps; EC Kinases; EC oncogene protein, Fujinami sarcoma virus; EC 3.6.1.-/rab GTP-Binding Proteins

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