| Subcellular localization of HMFG2 in breast carcinomas: an immunohistochemical and immunoelectron microscopic study. | |
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MedLine Citation:
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PMID: 1369793 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Malignant transformation of human cells is associated with morphological and biochemical alterations. We have studied the distribution and pattern of staining of HMFG2 (human milk fat globulin) in normal breast, benign breast lesions, and 137 primary and metastatic breast carcinomas. Immunohistochemical staining was performed with an antibody to HMFG2 using the indirect peroxidase technique. Three patterns of staining were noted: 1) secretion and luminal staining (in normal breast, most benign breast lesions and some breast carcinomas); 2) plasma membrane staining (in breast carcinomas); 3) intracytoplasmic staining (in breast carcinomas). Immunoelectron microscopy was also performed on normal breast, infiltrating duct, and lobular carcinomas. Immunoelectron microscopy showed localization of the gold particles on the electron dense granules of the HMFG2 protein. These were localized along the surface of the extracytoplasmic lumina in normal breast ducts/acini and breast carcinomas, whereas localization was also noted within the intracytoplasmic lumina in cancer cells only. These results show that there is altered localization of milk fat globulin in breast cancer cells associated with membrane internalization and formation of intracytoplasmic lumina. This contributes to the understanding of the phenotypic alterations associated with malignant transformation in breast cancer. |
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Authors:
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W M Hanna; H J Kahn; S E Zive; M Shackleton; L Andrighetti |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc Volume: 5 ISSN: 0893-3952 ISO Abbreviation: Mod. Pathol. Publication Date: 1992 Nov |
Date Detail:
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Created Date: 1995-09-07 Completed Date: 1995-09-07 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8806605 Medline TA: Mod Pathol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 603-6 Citation Subset: IM |
Affiliation:
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Department of Pathology, Women's College Hospital, University of Toronto, Ontario, Canada. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Breast Diseases
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metabolism,
pathology Breast Neoplasms / metabolism*, pathology Carcinoma / metabolism*, pathology Humans Immunohistochemistry Membrane Glycoproteins / metabolism* Microscopy, Electron Microscopy, Immunoelectron Mucin-1 Mucins / metabolism* Neoplasm Proteins / metabolism Reference Values Subcellular Fractions / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Membrane Glycoproteins; 0/Mucin-1; 0/Mucins; 0/Neoplasm Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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