| Subcellular localisation of di- and tripeptidases in guinea pig and rat enterocytes. | |
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MedLine Citation:
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PMID: 465509 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Enterocytes were isolated from rat and guinea pig jejunum and subcellular fractions were prepared by density gradient centrifugation. Gradient fractions were assayed for principal organelle marker enzymes and for di- and tripeptidases. The hydrolases showed a dual localisation with both brush border and cytosol components. In the rat, approximately equal portions of dipeptidase activities were found in the two fractions but, in the guinea pig, three times more activity were found in the two fractions but, in the guinea pig, three times more activity was found in the soluble than in the brush border fractions. Cytosol components in the rat were markedly inhibited by p-hydroxymercuribenzoate. In both species tripeptidase, leucyl-2-naphthylamidases and gamma-glutamyltransferase activities were found predominantly in the brush border fractions. |
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Authors:
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G P Wells; J A Nicholson; T J Peters |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 569 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1979 Jul |
Date Detail:
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Created Date: 1979-10-26 Completed Date: 1979-10-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 82-8 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Fractionation Centrifugation, Density Gradient Dipeptidases / isolation & purification* Guinea Pigs Jejunum / enzymology*, ultrastructure Male Microvilli / enzymology Mitochondria / enzymology Oligopeptides Peptide Hydrolases / isolation & purification* Rats Species Specificity |
| Chemical | |
Reg. No./Substance:
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0/Oligopeptides; EC 3.4.-/Peptide Hydrolases; EC 3.4.13.-/Dipeptidases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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