Document Detail

Subcellular electrolyte shifts during in vitro myocardial ischemia and reperfusion.
MedLine Citation:
PMID:  3177681     Owner:  NLM     Status:  MEDLINE    
Isolated perfused rabbit right ventricular wall was studied with electron probe microanalysis (EPMA) under three conditions: 1) control (37 degrees C, 1.2 Hz), 2) 60 min global ischemia, and 3) ischemia plus 5 min of reperfusion. After 60 min of ischemia, only one cell population was evident; the variance of intracellular electrolyte concentrations was the same as in controls. When compared with controls, there was no change in Ca concentration within any region of the cell, but mitochondria were swollen with K-rich fluid. Two cell populations were evident after 5 min of reperfusion. The severely injured cells were markedly swollen, exhibited hypercontraction bands, and had electrolyte profiles similar to extracellular fluid. The moderately injured cells were normal in appearance, still retained electrolyte gradients, but had elevated Na and Cl concentrations in all compartments. Cell Ca did not increase in the moderately injured cells, but the region of the cell containing the sarcoplasmic reticulum (SR) lost 90% of its Ca. Accompanying this loss were large increases in myofibrillar and mitochondrial Ca concentration. It appears that release of SR Ca, loss of SR Ca-accumulating capacity, and increased intracellular Na are the principal electrolyte shifts in functional cells during early reperfusion.
L G Walsh; J M Tormey
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The American journal of physiology     Volume:  255     ISSN:  0002-9513     ISO Abbreviation:  Am. J. Physiol.     Publication Date:  1988 Oct 
Date Detail:
Created Date:  1988-11-09     Completed Date:  1988-11-09     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370511     Medline TA:  Am J Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  H917-28     Citation Subset:  IM    
Department of Physiology, School of Medicine, University of California, Los Angeles 90024.
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MeSH Terms
Coronary Disease / metabolism*
Disease Models, Animal
Electrolytes / metabolism*
Electron Probe Microanalysis
Heart Ventricles / metabolism
Microscopy, Electron
Microscopy, Electron, Scanning
Myocardium / metabolism*,  ultrastructure
Myofibrils / metabolism,  ultrastructure
Reference Values
Subcellular Fractions / metabolism
Grant Support
Reg. No./Substance:

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