Document Detail

Subcellular distribution of okadaic acid in the digestive gland of Mytilus galloprovincialis: first evidences of lipoprotein binding to okadaic acid.
MedLine Citation:
PMID:  19646464     Owner:  NLM     Status:  MEDLINE    
The subcellular distribution of okadaic acid, the main diarrhetic shellfish poisoning (DSP) toxin, in the cells of the digestive gland of the mussel Mytilus galloprovincialis was studied. By means of differential centrifugation, ultrafiltration and extraction with methanol, it was found that most okadaic acid was stored in the cytosol. Notwithstanding only a small proportion of the total toxin was found to be in free form, being most of it bound to a soluble cellular compound with a molecular mass which ranged from 30 to 300 kDa. A series of fractionations of samples digested with a protease, a lipase, and amylase suggested that the component to which okadaic acid is bound is a high density lipoprotein. A new fractionation after digestion with a protein lipase additionally supports the previous conclusion.
Araceli E Rossignoli; Juan Blanco
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-07-29
Journal Detail:
Title:  Toxicon : official journal of the International Society on Toxinology     Volume:  55     ISSN:  1879-3150     ISO Abbreviation:  Toxicon     Publication Date:    2010 Feb-Mar
Date Detail:
Created Date:  2010-01-29     Completed Date:  2010-04-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1307333     Medline TA:  Toxicon     Country:  England    
Other Details:
Languages:  eng     Pagination:  221-6     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Elsevier Ltd. All rights reserved.
Centro de Investigaci?ns Mari?as, Pedras de Cor?n, s/n. Apdo. 13, 36620 Vilanova de Arousa (Pontevedra), Spain.
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MeSH Terms
Amylases / chemistry
Carcinogens / metabolism*
Chromatography, High Pressure Liquid
Digestive System / metabolism*
Exocrine Glands / metabolism*
Lipoprotein Lipase / chemistry
Lipoproteins / metabolism*
Mytilus / metabolism*
Okadaic Acid / metabolism*
Peptide Hydrolases / chemistry
Protease Inhibitors / chemistry
Protein Binding
Spectrometry, Mass, Electrospray Ionization
Subcellular Fractions / chemistry,  metabolism*
Reg. No./Substance:
0/Carcinogens; 0/Lipoproteins; 0/Protease Inhibitors; 78111-17-8/Okadaic Acid; EC Lipase; EC 3.2.1.-/Amylases; EC 3.4.-/Peptide Hydrolases

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