| Subcellular distribution of okadaic acid in the digestive gland of Mytilus galloprovincialis: first evidences of lipoprotein binding to okadaic acid. | |
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MedLine Citation:
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PMID: 19646464 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The subcellular distribution of okadaic acid, the main diarrhetic shellfish poisoning (DSP) toxin, in the cells of the digestive gland of the mussel Mytilus galloprovincialis was studied. By means of differential centrifugation, ultrafiltration and extraction with methanol, it was found that most okadaic acid was stored in the cytosol. Notwithstanding only a small proportion of the total toxin was found to be in free form, being most of it bound to a soluble cellular compound with a molecular mass which ranged from 30 to 300 kDa. A series of fractionations of samples digested with a protease, a lipase, and amylase suggested that the component to which okadaic acid is bound is a high density lipoprotein. A new fractionation after digestion with a protein lipase additionally supports the previous conclusion. |
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Authors:
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Araceli E Rossignoli; Juan Blanco |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-07-29 |
Journal Detail:
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Title: Toxicon : official journal of the International Society on Toxinology Volume: 55 ISSN: 1879-3150 ISO Abbreviation: Toxicon Publication Date: 2010 Feb-Mar |
Date Detail:
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Created Date: 2010-01-29 Completed Date: 2010-04-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1307333 Medline TA: Toxicon Country: England |
Other Details:
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Languages: eng Pagination: 221-6 Citation Subset: IM |
Copyright Information:
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Copyright 2009 Elsevier Ltd. All rights reserved. |
Affiliation:
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Centro de Investigaci?ns Mari?as, Pedras de Cor?n, s/n. Apdo. 13, 36620 Vilanova de Arousa (Pontevedra), Spain. ara@cimacoron.org |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amylases
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chemistry Animals Carcinogens / metabolism* Chromatography, High Pressure Liquid Digestive System / metabolism* Exocrine Glands / metabolism* Hydrolysis Lipoprotein Lipase / chemistry Lipoproteins / metabolism* Mytilus / metabolism* Okadaic Acid / metabolism* Peptide Hydrolases / chemistry Protease Inhibitors / chemistry Protein Binding Spectrometry, Mass, Electrospray Ionization Subcellular Fractions / chemistry, metabolism* Ultrafiltration |
| Chemical | |
Reg. No./Substance:
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0/Carcinogens; 0/Lipoproteins; 0/Protease Inhibitors; 78111-17-8/Okadaic Acid; EC 3.1.1.34/Lipoprotein Lipase; EC 3.2.1.-/Amylases; EC 3.4.-/Peptide Hydrolases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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