Document Detail


Subcellular distribution and immunocytochemical localization of Na,K-ATPase subunit isoforms in human skeletal muscle.
MedLine Citation:
PMID:  7711835     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The expression of Na,K-ATPase isoforms was investigated in human skeletal muscle membranes isolated by subcellular fractionation. The alpha 1, alpha 2, alpha 3 and beta 1 subunits were detectable in membranes prepared from the human soleus muscle. The alpha 1 subunit was largely detected in a fraction enriched with plasma membranes (PM), its abundance in an intracellular membrane fraction (IM) accounted for only 4% of that in the PM fraction. No alpha 1 subunits were detected in membranes of sarcoplasmic reticulum (SR) origin. The PM and IM fractions were enriched with alpha 2 subunits which were less abundant in the SR-enriched fraction. The abundance of alpha 2 molecules within the IM fraction was about 75% of that in the PM fraction when the total protein content for the two fractions was taken into account. Immunocytochemical studies confirmed the localization of the alpha 1 subunit to the muscle cell surface. The alpha 2 subunit was also found to be present in the cell surface but the observation that alpha 2 immunofluorescence was diffusely dispersed throughout the muscle fibre indicated that it was also present intracellularly, consistent with its biochemical localization in the PM and IM membrane fractions. The alpha 3 subunit was detected largely in the PM fraction but the lack of good antibodies to this isoform precluded an analysis of its immunocytochemical localization. The beta 1 subunit was enriched in the PM fraction but was also detected to a modest extent in the IM.(ABSTRACT TRUNCATED AT 250 WORDS)
Authors:
H S Hundal; D L Maxwell; A Ahmed; F Darakhshan; Y Mitsumoto; A Klip
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Molecular membrane biology     Volume:  11     ISSN:  0968-7688     ISO Abbreviation:  Mol. Membr. Biol.     Publication Date:    1994 Oct-Dec
Date Detail:
Created Date:  1995-05-16     Completed Date:  1995-05-16     Revised Date:  2009-09-29    
Medline Journal Info:
Nlm Unique ID:  9430797     Medline TA:  Mol Membr Biol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  255-62     Citation Subset:  IM    
Affiliation:
Department of Anatomy and Physiology, University of Dundee, UK.
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MeSH Terms
Descriptor/Qualifier:
Antibodies
Antibody Specificity
Humans
Immunoblotting
Immunohistochemistry
Isoenzymes / analysis*,  immunology
Macromolecular Substances
Membranes / chemistry,  enzymology
Muscle, Skeletal / chemistry,  enzymology*
Sodium-Potassium-Exchanging ATPase / analysis*,  immunology
Subcellular Fractions / chemistry,  enzymology
Grant Support
ID/Acronym/Agency:
//Wellcome Trust
Chemical
Reg. No./Substance:
0/Antibodies; 0/Isoenzymes; 0/Macromolecular Substances; EC 3.6.3.9/Sodium-Potassium-Exchanging ATPase

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