| Subcellular distribution of esterases in the bovine eye. | |
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MedLine Citation:
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PMID: 7187643 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The distribution of esterases within a cell can influence the rate and extent at which drugs containing ester linkages would be hydrolyzed. The objective of this study was to evaluate the subcellular distribution of esterases in the bovine eye. Ocular tissue homogenates were subjected to differential centrifugation to yield mitochondrial, microsomal and cytoplasmic fractions. Each fraction was then incubated with 1- or 2-naphthyl esters and its esterase activity determined. No esterase activity was detected in the mitochondrial fraction. For the corneal epithelium and iris-ciliary body about 80% of the esterase activity in the tissue homogenates, on a per milligram protein basis, was associated with the microsomes, the remainder was associated with the cytoplasm. Of the tissues studied, the iris-ciliary body had the highest esterase activity, 15 and 7 times that in the corneal epithelium and corneal stroma, respectively. The corneal endothelium was devoid of esterase activity. In comparison with the rabbit eye, the specific esterase activity in the bovine eye was lower. Surprisingly, unlike the rabbit, the corneal epithelium of the bovine eye was enzymatically less active than the stroma. Based on these preliminary data, it was concluded that the esterase activity varies with the tissue and that within a cell the esterases were distributed between the cytoplasm and the microsomes according to a ratio specific for a tissue. |
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Authors:
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V H Lee; D S Iimoto; K A Takemoto |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Current eye research Volume: 2 ISSN: 0271-3683 ISO Abbreviation: Curr. Eye Res. Publication Date: 1982-1983 |
Date Detail:
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Created Date: 1984-02-14 Completed Date: 1984-02-14 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8104312 Medline TA: Curr Eye Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 869-76 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cattle Ciliary Body / enzymology Cornea / enzymology Cytoplasm / enzymology Epithelium / enzymology Esterases / analysis* Eye / enzymology* Iris / enzymology Microsomes / enzymology Rabbits |
| Grant Support | |
ID/Acronym/Agency:
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EY-03816/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
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EC 3.1.-/Esterases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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