Document Detail


Subcellular biodistribution of sodium borocaptate (BSH: Na2B12H11SH) in a rat glioma model in boron neutron capture therapy.
MedLine Citation:
PMID:  12241106     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mercaptoundecahydrododecaborate (Na2B12H111SH, sodium borocaptate or 'BSH') has been used clinically as a boron compound for boron neutron capture therapy (BNCT) in patients with malignant glioma in Japan and Europe. Boron-10 is known to accumulate selectively only in brain tumor cells. This work was aimed to clarify the subcellular biodistribution of BSH in a rat glioma model using immunohistochemical approach. Wistar rats were used for this experiment. An intracerebral injection of 5.0 x 10(6) C6 glioma cells was introduced into the region of cerebral hemisphere. Fifty milligrams of "'B/kg BSH was infused intravenously two weeks after implantation. Host rats were divided into six groups according to the sampling time: 1, 4, 8, 16, 24 and 48 h after the start of BSH infusion. Immunohistochemical study was carried out using anti-BSH antibody. Boron was already found in a whole cell 1 h after BSH infusion, and then seemed to collect in a cell nuclei around 8-16 h after infusion. It was still recognized in tumor cell 48 h after infusion. This study supports the following hypothesis on selective boron uptake in a tumor. BSH can pass through the disrupted blood-brain barrier (BBB) easily and can come in contact with tumor cells; there, BSH can bind on the extracellular surface of plasma membrane to choline residues. After binding to the plasma membrane, boron with choline residues may be internalized into the cell by endocytic pathways and eventually travel to cell nuclei, and then stay there for a long time.
Authors:
Teruyoshi Kageji; Shinji Nagahiro; Birte Otersen; Detlef Gabel; Manekazu Nakaichi; Yoshinobu Nakagawa
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of neuro-oncology     Volume:  59     ISSN:  0167-594X     ISO Abbreviation:  J. Neurooncol.     Publication Date:  2002 Sep 
Date Detail:
Created Date:  2002-09-20     Completed Date:  2003-03-28     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8309335     Medline TA:  J Neurooncol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  135-42     Citation Subset:  IM    
Affiliation:
Department of Neurological Surgery, School of Medicine, The University of Tokushima, Japan. kageji@clin.med.tokushima-u.ac.jp
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MeSH Terms
Descriptor/Qualifier:
Animals
Borohydrides / administration & dosage,  pharmacokinetics*
Boron / analysis
Boron Neutron Capture Therapy* / methods
Brain Neoplasms / chemistry,  metabolism*
Glioma / chemistry,  metabolism*
Immunohistochemistry
Infusions, Intravenous
Injections, Intraventricular
Neoplasm Transplantation
Rats
Rats, Wistar
Subcellular Fractions / chemistry
Sulfhydryl Compounds / administration & dosage,  pharmacokinetics*
Time Factors
Tissue Distribution
Chemical
Reg. No./Substance:
0/Borohydrides; 0/Sulfhydryl Compounds; 12294-22-3/mercaptoundecahydrododecaborate; 7440-42-8/Boron

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