| Subcellular localization and role of lipin-1 in human macrophages. | |
| | |
MedLine Citation:
|
PMID: 21478406 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The lipins have been described as metabolic enzymes that regulate lipid biosynthesis and also signaling processes by controlling the cellular concentration of bioactive lipids, phosphatidic acid, and diacylgycerol. In the present work we have studied the subcellular localization and role of lipin-1 in human monocyte-derived macrophages. Human macrophages express lipin-1 isoforms α and β. A transfected lipin-1α-enhanced GFP construct associates with membranes of cellular organelles that can be stained with Nile Red. Colocalization experiments with lipid droplet (LD)-specific proteins such as adipophilin/adipose differentiation-related protein/perilipin 2 or TIP47/perilipin 3 show that both proteins colocalize with lipin-1α in the same cellular structures. Reduction of the expression levels of lipin-1 by small interfering RNA technology does not impair triacylglycerol biosynthesis but reduces the size of LDs formed in response to oleic acid. In agreement with these data, peritoneal macrophages from animals that carry a mutation in the Lpin-1 gene (fld animals) also produce less and smaller LDs in response to oleic acid. Mass spectrometry determinations demonstrate that the fatty acid composition of triacylglycerol in isolated LDs from lipin-1-deficient cells differs from that of control cells. Moreover, activation of cytosolic group IVA phospholipase A(2)α, a proinflammatory enzyme that is also involved in LD biogenesis, is also compromised in lipin-1-deficient cells. Collectively, these data suggest that lipin-1 associates with LDs and regulates the activation of cytosolic group IVA phospholipase A(2)α in human monocyte-derived macrophages. |
| | |
Authors:
|
Martín Valdearcos; Esperanza Esquinas; Clara Meana; Luis Gil-de-Gómez; Carlos Guijas; Jesús Balsinde; María A Balboa |
Related Documents
:
|
11842396 - Advances in classification and therapy of indolent b-cell malignancies. 15367606 - Human t-cell leukemia virus type 1 (htlv-1) and htlv-2 tax oncoproteins modulate cell c... 9350436 - Molecular mechanisms of apoptosis in hl-60 cells induced by a nitric oxide-releasing co... 21531336 - Linking lipid metabolism to the innate immune response in macrophages through sterol re... 11921336 - Initiation of apoptosis in the developing avian outflow tract myocardium. 9475356 - Differential regulation of il-8 by il-1beta and tnfalpha in hyaline membrane disease. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-04-08 |
Journal Detail:
|
Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 186 ISSN: 1550-6606 ISO Abbreviation: J. Immunol. Publication Date: 2011 May |
Date Detail:
|
Created Date: 2011-05-04 Completed Date: 2011-07-05 Revised Date: 2011-10-18 |
Medline Journal Info:
|
Nlm Unique ID: 2985117R Medline TA: J Immunol Country: United States |
Other Details:
|
Languages: eng Pagination: 6004-13 Citation Subset: AIM; IM |
Affiliation:
|
Instituto de Biología y Genética Molecular, Consejo Superior de Investigaciones Científicas, 47003 Valladolid, Spain. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals DNA-Binding Proteins / metabolism Group IV Phospholipases A2 / genetics, metabolism* Humans Immunoblotting Intracellular Membranes / metabolism Intracellular Signaling Peptides and Proteins / metabolism Lipid Metabolism Lipids / analysis Macrophages / enzymology, metabolism* Mass Spectrometry Membrane Proteins / metabolism Mice Mice, Inbred BALB C Mutation Nuclear Proteins / genetics, metabolism* Oleic Acid / pharmacology Oxazines Polymerase Chain Reaction Pregnancy Proteins / metabolism Protein Isoforms / genetics, metabolism RNA, Small Interfering Triglycerides / biosynthesis |
| Chemical | |
Reg. No./Substance:
|
0/DNA-Binding Proteins; 0/Intracellular Signaling Peptides and Proteins; 0/Lipids; 0/Membrane Proteins; 0/Nuclear Proteins; 0/Oxazines; 0/PLIN3 protein, human; 0/Pregnancy Proteins; 0/Protein Isoforms; 0/RNA, Small Interfering; 0/Triglycerides; 0/perilipin 2; 112-80-1/Oleic Acid; 7385-67-3/nile red; EC 3.1.1.4/Group IV Phospholipases A2; EC 3.1.3.4/LPIN1 protein, human |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Promyelocytic Leukemia Zinc Finger Turns on the Effector T Cell Program without Requirement for Agon...
Next Document: H2O2 preconditioning modulates phase II enzymes through p38 MAPK and PI3K/Akt activation.