Document Detail

Subcellular localization of legionella Dot/Icm effectors.
MedLine Citation:
PMID:  23150406     Owner:  NLM     Status:  MEDLINE    
The translocation of effector proteins by the Dot/Icm type IV secretion system is central to the ability of Legionella pneumophila to persist and replicate within eukaryotic cells. The subcellular localization of translocated Dot/Icm proteins in host cells provides insight into their function. Through co-staining with host cell markers, effector proteins may be localized to specific subcellular compartments and membranes, which frequently reflects their host cell target and mechanism of action. In this chapter, we describe protocols to (1) localize effector proteins within cells by ectopic expression using green fluorescent protein fusions and (2) localize effector proteins within infected cells using epitope-tagged effector proteins and immuno-fluorescence microscopy.
Adam J Vogrin; Aurelie Mousnier; Gad Frankel; Elizabeth L Hartland
Related Documents :
12440956 - Factor h family proteins: on complement, microbes and human diseases.
21420516 - Identification of nacl stress-responsive apoplastic proteins in rice shoot stems by 2d-...
24004886 - Ph shift assembly of adenoviral serotype 5 capsid protein nanosystems for enhanced deli...
24319736 - Relationship between the argyrophilic proteins of the nucleolar organizer region and st...
9336836 - Structural heterogeneity of the various forms of apomyoglobin: implications for protein...
2518356 - Immunochemical comparison of pertussis toxin substrates in brain and peripheral tissues.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  954     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-15     Completed Date:  2013-04-19     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  333-44     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Bacterial Proteins / genetics,  metabolism*
Bacterial Secretion Systems*
Cloning, Molecular
Gene Expression
Green Fluorescent Proteins / genetics,  metabolism
HEK293 Cells
Legionella / genetics,  metabolism*
Legionella pneumophila / metabolism
Microscopy, Fluorescence
Plasmids / genetics
Protein Transport
Recombinant Fusion Proteins / genetics,  metabolism
Grant Support
G1001729//Medical Research Council
Reg. No./Substance:
0/Bacterial Proteins; 0/Recombinant Fusion Proteins; 147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Methods for determining protein translocation by the Legionella pneumophila Dot/Icm type IV secretio...
Next Document:  Purification and Characterization of Legionella U-Box-Type E3 Ubiquitin Ligase.