Document Detail


Subcellular localization of legionella Dot/Icm effectors.
MedLine Citation:
PMID:  23150406     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The translocation of effector proteins by the Dot/Icm type IV secretion system is central to the ability of Legionella pneumophila to persist and replicate within eukaryotic cells. The subcellular localization of translocated Dot/Icm proteins in host cells provides insight into their function. Through co-staining with host cell markers, effector proteins may be localized to specific subcellular compartments and membranes, which frequently reflects their host cell target and mechanism of action. In this chapter, we describe protocols to (1) localize effector proteins within cells by ectopic expression using green fluorescent protein fusions and (2) localize effector proteins within infected cells using epitope-tagged effector proteins and immuno-fluorescence microscopy.
Authors:
Adam J Vogrin; Aurelie Mousnier; Gad Frankel; Elizabeth L Hartland
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  954     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-15     Completed Date:  2013-04-19     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  333-44     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / genetics,  metabolism*
Bacterial Secretion Systems*
Cloning, Molecular
Gene Expression
Green Fluorescent Proteins / genetics,  metabolism
HEK293 Cells
Humans
Legionella / genetics,  metabolism*
Legionella pneumophila / metabolism
Microscopy, Fluorescence
Plasmids / genetics
Protein Transport
Recombinant Fusion Proteins / genetics,  metabolism
Transfection
Grant Support
ID/Acronym/Agency:
G1001729//Medical Research Council
Chemical
Reg. No./Substance:
0/Bacterial Proteins; 0/Recombinant Fusion Proteins; 147336-22-9/Green Fluorescent Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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