Document Detail


Subarachnoid hemorrhage and endothelial L-arginine pathway in small brain stem arteries in dogs.
MedLine Citation:
PMID:  8446976     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND AND PURPOSE: Experiments were designed to determine the effect of subarachnoid hemorrhage on endothelium-dependent relaxations in small arteries of the brain stem. A "double-hemorrhage" canine model of the disease was used, and the presence of vasospasm in the basilar artery was confirmed by angiography. METHODS: Secondary branches of both untreated basilar arteries (inner diameter, 324 +/- 11 microns; n = 12) and arteries exposed to subarachnoid hemorrhage for 7 days (inner diameter, 328 +/- 12 microns; n = 12) were dissected and mounted on glass microcannulas in organ chambers. Changes in the intraluminal diameter of pressurized arteries were measured using a video dimension analyzer. RESULTS: In untreated arteries, 10(-11) to 10(-7) M vasopressin, 10(-10) to 10(-6) M bradykinin, and 10(-9) to 10(-6) M calcium ionophore A23187 caused endothelium-dependent relaxations. At 10(-6) and 3 x 10(-4) M the nitric oxide synthase inhibitor NG-nitro-L-arginine methyl ester (L-NAME) abolished relaxations to vasopressin and produced small but significant rightward shifts of the concentration-response curves to bradykinin and A23187. At 10(-3) M L-arginine prevented the inhibitory effect of L-NAME. Subarachnoid hemorrhage abolished relaxations to vasopressin but did not affect relaxations to bradykinin or A23187. CONCLUSIONS: These studies suggest that in small arteries of the brain stem vasopressin causes relaxations by activation of the endothelial L-arginine pathway. This mechanism of relaxation is selectively inhibited by subarachnoid hemorrhage. Preservation of endothelium-dependent relaxations to bradykinin and A23187 is consistent with the concept that small arteries are resistant to vasospasm after subarachnoid hemorrhage.
Authors:
Z S Katusić; J H Milde; F Cosentino; B S Mitrović
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Stroke; a journal of cerebral circulation     Volume:  24     ISSN:  0039-2499     ISO Abbreviation:  Stroke     Publication Date:  1993 Mar 
Date Detail:
Created Date:  1993-04-02     Completed Date:  1993-04-02     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0235266     Medline TA:  Stroke     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  392-9     Citation Subset:  IM    
Affiliation:
Department of Anesthesiology, Mayo Clinic, Rochester, Minn. 55905.
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MeSH Terms
Descriptor/Qualifier:
Animals
Arginine / analogs & derivatives,  antagonists & inhibitors,  pharmacology*
Arterioles / drug effects
Bradykinin / antagonists & inhibitors
Brain Stem / blood supply*
Calcimycin / antagonists & inhibitors
Disease Models, Animal
Dogs
Dose-Response Relationship, Drug
Endothelium, Vascular / physiopathology*
Female
Male
NG-Nitroarginine Methyl Ester
Subarachnoid Hemorrhage / physiopathology*
Vasodilation
Vasopressins / antagonists & inhibitors
Grant Support
ID/Acronym/Agency:
GM 44486/GM/NIGMS NIH HHS; HL 44116/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
11000-17-2/Vasopressins; 50903-99-6/NG-Nitroarginine Methyl Ester; 52665-69-7/Calcimycin; 58-82-2/Bradykinin; 74-79-3/Arginine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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