Document Detail


Sub-nanomolar detection of prostate-specific membrane antigen in synthetic urine by synergistic, dual-ligand phage.
MedLine Citation:
PMID:  23614709     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The sensitive detection of cancer biomarkers in urine could revolutionize cancer diagnosis and treatment. Such detectors must be inexpensive, easy to interpret, and sensitive. This report describes a bioaffinity matrix of viruses integrated into PEDOT films for electrochemical sensing of prostate-specific membrane antigen (PSMA), a prostate cancer biomarker. High sensitivity to PSMA resulted from synergistic action by two different ligands to PSMA on the same phage particle. One ligand was genetically encoded, and the secondary recognition ligand was chemically synthesized to wrap around the phage. The dual ligands result in a bidentate binder with high-copy, dense ligand display for enhanced PSMA detection through a chelate-based avidity effect. Biosensing with virus-PEDOT films provides a 100 pM limit of detection for PSMA in synthetic urine without requiring enzymatic or other amplification.
Authors:
Kritika Mohan; Keith C Donavan; Jessica A Arter; Reginald M Penner; Gregory A Weiss
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2013-05-13
Journal Detail:
Title:  Journal of the American Chemical Society     Volume:  135     ISSN:  1520-5126     ISO Abbreviation:  J. Am. Chem. Soc.     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-05-22     Completed Date:  2013-12-24     Revised Date:  2014-05-23    
Medline Journal Info:
Nlm Unique ID:  7503056     Medline TA:  J Am Chem Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7761-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Alkynes / chemistry,  diagnostic use*
Antigens, Surface / urine*
Azides / chemistry,  diagnostic use*
Bacteriophages / chemistry,  genetics
Biosensing Techniques / methods*
Cyclization
Glutamate Carboxypeptidase II / urine*
Humans
Ligands
Limit of Detection
Male
Models, Molecular
Molecular Structure
Prostatic Neoplasms / urine*
Sulfhydryl Compounds / chemistry,  diagnostic use*
Tumor Markers, Biological / urine*
Grant Support
ID/Acronym/Agency:
1 R43 AI074163/AI/NIAID NIH HHS; R01 CA133592/CA/NCI NIH HHS; R01 CA133592-01/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Alkynes; 0/Antigens, Surface; 0/Azides; 0/Ligands; 0/Sulfhydryl Compounds; 0/Tumor Markers, Biological; EC 3.4.17.21/Glutamate Carboxypeptidase II; EC 3.4.17.21/glutamate carboxypeptidase II, human
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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