Document Detail

Sub-nanomolar detection of prostate-specific membrane antigen in synthetic urine by synergistic, dual-ligand phage.
MedLine Citation:
PMID:  23614709     Owner:  NLM     Status:  MEDLINE    
The sensitive detection of cancer biomarkers in urine could revolutionize cancer diagnosis and treatment. Such detectors must be inexpensive, easy to interpret, and sensitive. This report describes a bioaffinity matrix of viruses integrated into PEDOT films for electrochemical sensing of prostate-specific membrane antigen (PSMA), a prostate cancer biomarker. High sensitivity to PSMA resulted from synergistic action by two different ligands to PSMA on the same phage particle. One ligand was genetically encoded, and the secondary recognition ligand was chemically synthesized to wrap around the phage. The dual ligands result in a bidentate binder with high-copy, dense ligand display for enhanced PSMA detection through a chelate-based avidity effect. Biosensing with virus-PEDOT films provides a 100 pM limit of detection for PSMA in synthetic urine without requiring enzymatic or other amplification.
Kritika Mohan; Keith C Donavan; Jessica A Arter; Reginald M Penner; Gregory A Weiss
Related Documents :
19697359 - Frequent pik3ca gene amplification and its clinical significance in colorectal cancer.
17071139 - Microrna expression and function in cancer.
12492109 - Relationship of xist expression and responses of ovarian cancer to chemotherapy.
24939299 - Expression of nerve growth factor and hypoxia inducible factor-1α and its correlation ...
11745679 - Differential expression of vesicular monoamine transporter (vmat) 1 and 2 in gastrointe...
16676859 - Increased epithelial cadherin expression among japanese intestinal-type gastric cancers...
11824739 - Breast cancer: education, counseling, and adjustment--a pilot study.
10469169 - New strategies in anti-vascular cancer therapy.
19761689 - Human papillomavirus type 16 exists in bacteria isolated from cervical cancer biopsies.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2013-05-13
Journal Detail:
Title:  Journal of the American Chemical Society     Volume:  135     ISSN:  1520-5126     ISO Abbreviation:  J. Am. Chem. Soc.     Publication Date:  2013 May 
Date Detail:
Created Date:  2013-05-22     Completed Date:  2013-12-24     Revised Date:  2014-05-23    
Medline Journal Info:
Nlm Unique ID:  7503056     Medline TA:  J Am Chem Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  7761-7     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Alkynes / chemistry,  diagnostic use*
Antigens, Surface / urine*
Azides / chemistry,  diagnostic use*
Bacteriophages / chemistry,  genetics
Biosensing Techniques / methods*
Glutamate Carboxypeptidase II / urine*
Limit of Detection
Models, Molecular
Molecular Structure
Prostatic Neoplasms / urine*
Sulfhydryl Compounds / chemistry,  diagnostic use*
Tumor Markers, Biological / urine*
Grant Support
1 R43 AI074163/AI/NIAID NIH HHS; R01 CA133592/CA/NCI NIH HHS; R01 CA133592-01/CA/NCI NIH HHS
Reg. No./Substance:
0/Alkynes; 0/Antigens, Surface; 0/Azides; 0/Ligands; 0/Sulfhydryl Compounds; 0/Tumor Markers, Biological; EC Carboxypeptidase II; EC carboxypeptidase II, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Factors impacting echocardiographic imaging after the Fontan procedure: a report from the pediatric ...
Next Document:  The Edmonton Classification System for Cancer Pain: Comparison of Pain Classification Features and P...