Document Detail


Sub-chronic effects of nitrate in drinking water on red-legged partridge (Alectoris rufa): oxidative stress and T-cell mediated immune function.
MedLine Citation:
PMID:  20382380     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In order to evaluate the effects of nitrates on birds, we have exposed captive red-legged partridges to nitrates concentrations of 0 (control), 100 (dwell water in farming areas) or 500 mg/l (fertirrigation level). The cellular immune response, plasma biochemistry, methemoglobin concentration (metHb), and oxidative stress biomarkers in blood and tissues were studied after two weeks of exposure. Several blood parameters such as aspartate aminotransferase, creatinine phosphokinase and lactate dehydrogenase activities and magnesium level decreased with nitrate exposure, whereas alkaline phosphatase activity and creatinine level increased. The oxidant effect of nitrates was evidenced by the increase in blood metHb, accompanied by the lipid peroxidation of red blood cells, the increased levels of oxidized glutathione (GSH) in liver, and the generation of oxidative DNA damage in plasma lymphocytes. GSH in erythrocytes was negatively correlated with blood metHb. The cellular immune function was slightly lower at partridges exposed to nitrates. These results suggest that adverse effects of nitrates on birds occur at concentrations potentially present in the field.
Authors:
Jaime Rodríguez-Estival; Mónica Martínez-Haro; M A Paz Martín-Hernando; Rafael Mateo
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-04-10
Journal Detail:
Title:  Environmental research     Volume:  110     ISSN:  1096-0953     ISO Abbreviation:  Environ. Res.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-06-11     Completed Date:  2010-07-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0147621     Medline TA:  Environ Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  469-75     Citation Subset:  IM    
Copyright Information:
2010 Elsevier Inc. All rights reserved.
Affiliation:
Instituto de Investigación en Recursos Cinegéticos, IREC (CSIC, UCLM, JCCM), Ronda de Toledo s/n, 13071 Ciudad Real, Spain.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Markers / blood
Comet Assay
DNA Damage
Dose-Response Relationship, Drug
Drinking
Female
Galliformes / immunology*,  metabolism
Immunity, Cellular / drug effects
Liver / metabolism
Methemoglobin / metabolism
Nitrates / administration & dosage,  toxicity*
Oxidative Stress*
T-Lymphocytes / drug effects*
Tocopherols / blood,  metabolism
Vitamin A / blood,  metabolism
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Nitrates; 11103-57-4/Vitamin A; 1406-66-2/Tocopherols; 9008-37-1/Methemoglobin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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