| Styrene-7,8-oxide activates a complex apoptotic response in neuronal PC12 cell line. | |
| | |
MedLine Citation:
|
PMID: 12663515 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Styrene-7,8-oxide (SO), the major in vivo metabolite of styrene, one of the most important plastic monomers worldwide, is classified as carcinogenic in humans and animals. Although the toxic effects of SO have been extensively documented in human lymphocytes, the molecular mechanisms responsible for SO-induced cell damage are still unknown. In the present study, we evaluated the effect of SO on growth and apoptosis, assessed by FACS and gel ladder analysis, in neuronal PC12 cell line. Our results demonstrate that SO triggered PC12 cell apoptosis in a dose- and time-dependent manner. PC12 apoptosis was associated with caspase-3 activation and modulation of the Bcl-2 family proteins. In addition, examination of the cytoskeleton showed that SO induced F-actin depolymerization and a rapid cell rounding before caspase-3 activation, suggesting that the changes in cell shape involving cytoskeletal structure are an early step in the apoptotic pathway. Therefore, SO triggers a complex apoptotic response consisting of a loss of cytoskeletal organization that precedes caspase-3 activation. These mechanisms may represent the molecular basis of the different SO sensitivity to tumor promotion among species and organs. |
| | |
Authors:
|
Mariarosaria Boccellino; Franca Cuccovillo; Maria Napolitano; Nicola Sannolo; Ciro Balestrieri; Antonio Acampora; Alfonso Giovane; Lucio Quagliuolo |
Related Documents
:
|
16140185 - Paclitaxel induces apoptosis via caspase-3 activation in human osteogenic sarcoma cells... 19766705 - Chlorophenols and chlorocatechols induce apoptosis in human lymphocytes (in vitro). 21980415 - Identification of a conserved anti-apoptotic protein that modulates the mitochondrial a... 18167185 - Histone deacetylase inhibitor trichostatin a induced caspase-independent apoptosis in h... 6607555 - Paradoxical effects of lps on the t-cell-independent type 2 anti-h-2 alloantigen antibo... 18537565 - Targeting toll-like receptor signaling pathways for design of novel immune therapeutics. |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Carcinogenesis Volume: 24 ISSN: 0143-3334 ISO Abbreviation: Carcinogenesis Publication Date: 2003 Mar |
Date Detail:
|
Created Date: 2003-03-28 Completed Date: 2003-04-30 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 8008055 Medline TA: Carcinogenesis Country: England |
Other Details:
|
Languages: eng Pagination: 535-40 Citation Subset: IM |
Affiliation:
|
Dipartimento di Biochimica e Biofisica, Seconda Università degli Studi di Napoli, Via Costantinopoli 16, I-80138 Napoli, Italy. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Apoptosis / drug effects* Blotting, Western Caspase 3 Caspases / metabolism Enzyme Activation Epoxy Compounds / pharmacology* Neurons / cytology, drug effects* PC12 Cells Rats |
| Chemical | |
Reg. No./Substance:
|
0/Epoxy Compounds; 96-09-3/styrene oxide; EC 3.4.22.-/CASP3 protein, human; EC 3.4.22.-/Casp3 protein, rat; EC 3.4.22.-/Caspase 3; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Associations between tissue-specific DNA alkylation, DNA repair and cell proliferation in the colon ...
Next Document: DNA repair protein MGMT protects against N-methyl-N-nitrosourea-induced conversion of benign into ma...