Document Detail


Studying recombination in mouse oocytes.
MedLine Citation:
PMID:  23138941     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Meiosis is the specialized cell division in sexually reproducing organisms in which haploid gametes are produced. Meiotic prophase I is the defining stage of meiosis, when pairing and synapsis occur between homologous chromosomes, concurrent with reciprocal recombination (or crossing over) events that arise between them. Any disruption of these events during prophase I can lead to improper segregation of homologous chromosomes which can cause severe birth defects in the resulting progeny, and this occurs with alarming frequency in human oocytes. Thus, while the pathways that regulate these events in prophase I are highly conserved in both males and females, the stringency with which these events are monitored and/or controlled appears to be dramatically lower in females. These observations underscore the need to examine and compare meiotic mechanisms across the sexes. However, the study of female meiosis is impeded by the early start of meiosis during fetal development and the very limited amount of ovarian tissue available for meiotic analyses. Here we describe three different techniques which are useful for meiotic prophase I analysis in mouse/human oocytes, ranging from early prophase I events through until the resolution of crossing over at the first and second meiotic divisions.
Authors:
Xianfei Sun; Paula E Cohen
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  957     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-09     Completed Date:  2013-04-12     Revised Date:  2013-12-13    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1-18     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Chromosomes, Mammalian / genetics
Cytogenetic Analysis / methods*
Female
Fetus / cytology,  metabolism
Fluorescent Antibody Technique, Indirect
Humans
Male
Meiotic Prophase I / genetics
Metaphase / genetics
Mice
Oocytes / cytology*,  metabolism*
Ovary / cytology,  metabolism
Recombination, Genetic*
Grant Support
ID/Acronym/Agency:
HD041012/HD/NICHD NIH HHS; R01 GM097263/GM/NIGMS NIH HHS; R01 HD041012/HD/NICHD NIH HHS; R56 HD041012/HD/NICHD NIH HHS

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