Document Detail

Studying meiosis-specific cohesins in mouse embryonic oocytes.
MedLine Citation:
PMID:  23138943     Owner:  NLM     Status:  MEDLINE    
Distinct meiotic cohesin complexes play fundamental roles in various meiosis-specific chromosomal events in spatiotemporally different manners during mammalian meiotic prophase. Immunostaining is one of the essential methods to study meiotic cohesin dynamics. For the study of cohesins in the meiotic prophase of oocytes, ovaries should be taken from the embryos during a very limited period before birth. Here we focus on some technical tips concerning the preparation of oocyte chromosome spreads for immunostaining. Further, we describe a method for chromosome fluorescence in situ hybridization (FISH) against immunostained oocytes.
Ji-hye Kim; Kei-ichiro Ishiguro; Nobuaki Kudo; Yoshinori Watanabe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  957     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2013  
Date Detail:
Created Date:  2012-11-09     Completed Date:  2013-04-12     Revised Date:  2014-02-20    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  47-57     Citation Subset:  IM    
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MeSH Terms
Cell Cycle Proteins / metabolism*
Chromosomal Proteins, Non-Histone / metabolism*
Chromosomes, Mammalian / genetics
Cytogenetic Analysis / methods*
Embryo, Mammalian / cytology*
In Situ Hybridization, Fluorescence
Meiosis / genetics*
Oocytes / cytology*,  metabolism*
Ovary / cytology,  embryology
Prophase / genetics
Grant Support
G0901046//Medical Research Council
Reg. No./Substance:
0/Cell Cycle Proteins; 0/Chromosomal Proteins, Non-Histone; 0/cohesins

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