| Studying ischemia and reperfusion in isolated neonatal rat ventricular myocytes using coverslip hypoxia. | |
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MedLine Citation:
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PMID: 18287679 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In vitro experimental models designed to study the effects of hypoxia and ischemia typically employ oxygen-depleted media and/or hypoxic chambers. These approaches, however, allow for metabolites to diffuse away into a large volume and may not replicate the local buildup of metabolic byproducts that occur in ischemic myocardium in vivo. Coverslip hypoxia (CSH) is a recently described method for studying hypoxia and ischemia derived from the byproducts and metabolites of contractile ventricular myocytes. Hence, this method is dependent on the purity and contractile activity of the isolated myocytes. We describe herein methods for isolating neonatal rat ventricular myocytes with these characteristics, as well as means for performing CSH, identifying viable and compromised myocytes after coverslipping, and tracking pH changes during CSH. |
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Authors:
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Kelly R Pitts; Christopher F Toombs |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Methods in molecular medicine Volume: 139 ISSN: 1543-1894 ISO Abbreviation: Methods Mol. Med. Publication Date: 2007 |
Date Detail:
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Created Date: 2008-02-21 Completed Date: 2008-10-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101123138 Medline TA: Methods Mol Med Country: United States |
Other Details:
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Languages: eng Pagination: 271-81 Citation Subset: IM |
Affiliation:
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Gilead Colorado, Inc., Molecular Pharmacology, Westminster, CO, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Anoxia / metabolism* Cell Culture Techniques* Cells, Cultured Heart Ventricles / cytology* Myocardial Ischemia / metabolism* Myocytes, Cardiac / cytology, physiology* Rats |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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