Document Detail

Studying ischemia and reperfusion in isolated neonatal rat ventricular myocytes using coverslip hypoxia.
MedLine Citation:
PMID:  18287679     Owner:  NLM     Status:  MEDLINE    
In vitro experimental models designed to study the effects of hypoxia and ischemia typically employ oxygen-depleted media and/or hypoxic chambers. These approaches, however, allow for metabolites to diffuse away into a large volume and may not replicate the local buildup of metabolic byproducts that occur in ischemic myocardium in vivo. Coverslip hypoxia (CSH) is a recently described method for studying hypoxia and ischemia derived from the byproducts and metabolites of contractile ventricular myocytes. Hence, this method is dependent on the purity and contractile activity of the isolated myocytes. We describe herein methods for isolating neonatal rat ventricular myocytes with these characteristics, as well as means for performing CSH, identifying viable and compromised myocytes after coverslipping, and tracking pH changes during CSH.
Kelly R Pitts; Christopher F Toombs
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Methods in molecular medicine     Volume:  139     ISSN:  1543-1894     ISO Abbreviation:  Methods Mol. Med.     Publication Date:  2007  
Date Detail:
Created Date:  2008-02-21     Completed Date:  2008-10-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101123138     Medline TA:  Methods Mol Med     Country:  United States    
Other Details:
Languages:  eng     Pagination:  271-81     Citation Subset:  IM    
Gilead Colorado, Inc., Molecular Pharmacology, Westminster, CO, USA.
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MeSH Terms
Animals, Newborn
Anoxia / metabolism*
Cell Culture Techniques*
Cells, Cultured
Heart Ventricles / cytology*
Myocardial Ischemia / metabolism*
Myocytes, Cardiac / cytology,  physiology*

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