Document Detail

Studying the effects of actin cytoskeletal destabilization on cell cycle by cofilin overexpression.
MedLine Citation:
PMID:  16118410     Owner:  NLM     Status:  MEDLINE    
The significance of actin cytoskeleton on cell growth was historically studied using toxic drugs, such as cytochalasin. However, it is possible that unpredictable effects of these agents may have influenced the reported observations. In our study, we have established a drug-free system using cofilin overexpression to investigate the relationship between actin filaments and cell cycle progression. Cofilin is a member of the actin depolymerization factor (ADF)/cofilin family, cofilin cDNA was cloned to a tetracycline-inducible gene expression vector and stably transfected to human lung cancer H1299 epithelial cells. Destabilization of actin filaments and morphological change was detected in cofilin overexpressing cells by actin analysis and microscopy, respectively. Measurements of growth rates showed that cell proliferation was retarded in cells with overexpressed cofilin. Also, cell cycle analysis showed that approx 90% of cofilin overexpressing cells were arrested in G1 phase, which is consistent with previous reports that drug-mediated disruption of actin filaments can cause G1 phase arrest. Taken together, cofilin overexpression cell model provides evidence that the effects of actin cytoskeletal destabilization on cell cycle progression can be studied using molecular approach instead of drug.
Yi-Jang Lee; Peter C Keng
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Molecular biotechnology     Volume:  31     ISSN:  1073-6085     ISO Abbreviation:  Mol. Biotechnol.     Publication Date:  2005 Sep 
Date Detail:
Created Date:  2005-08-24     Completed Date:  2005-12-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9423533     Medline TA:  Mol Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1-10     Citation Subset:  IM    
Department of Pathology and Laboratory Medicine, 9 Nothwood Ln, Middletown, CT 06457, USA.
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MeSH Terms
Actin Depolymerizing Factors / genetics,  physiology*
Blotting, Western
Cell Culture Techniques
Cell Cycle / physiology*
Cell Cycle Proteins / genetics*,  physiology
Cell Line, Tumor
Cell Movement
Cell Proliferation
Epithelial Cells / physiology*
Lung / cytology*
Lung Neoplasms
Microfilaments / genetics,  physiology*
Reg. No./Substance:
0/Actin Depolymerizing Factors; 0/Actins; 0/Cell Cycle Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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