Document Detail

Studying DNA translocation in nanocapillaries using single molecule fluorescence.
MedLine Citation:
PMID:  23284180     Owner:  NLM     Status:  Publisher    
We demonstrate simultaneous measurements of DNA translocation into glass nanopores using ionic current detection and fluorescent imaging. We verify the correspondence between the passage of a single DNA molecule through the nanopore and the accompanying characteristic ionic current blockage. By tracking the motion of individual DNA molecules in the nanocapillary perpendicular to the optical axis and using a model, we can extract an effective mobility constant for DNA in our geometry under high electric fields.
Vivek V Thacker; Sandip Ghosal; Silvia Hernández-Ainsa; Nicholas A W Bell; Ulrich F Keyser
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-11-30
Journal Detail:
Title:  Applied physics letters     Volume:  101     ISSN:  0003-6951     ISO Abbreviation:  Appl Phys Lett     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2013-1-3     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9881183     Medline TA:  Appl Phys Lett     Country:  -    
Other Details:
Languages:  ENG     Pagination:  223704     Citation Subset:  -    
Cavendish Laboratory, University of Cambridge, JJ Thompson Avenue, Cambridge CB3 0HE, United Kingdom.
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