| Study of the stability of a paramagnetic label linked to mesoporous silica surface in contact with rat mesothelial cells in culture. | |
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MedLine Citation:
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PMID: 9400695 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Stable radicals detectable by electron paramagnetic resonance (EPR) may be use in the investigation of early events in cell-particle toxicity. Piperidine-N-oxyl derivatives (nitroxides), covalently linked to the surface of a high surface area silica (used as model solid for the technique), served as probes in the investigation of the effects of incubation of silica particles with mesothelial cells. A mesoporous silica (MCM-41), prepared by precipitation from a micellar solution, was the most appropriate silica-based particle for this purpose, as its channels allow direct contact with small molecules but not with macromolecules. The cytotoxicity of this amorphous silica is very low, allowing relatively high particle loading in the cell cultures. Both the high surface area of the sample and the large amount of inorganic material extracted from the cell culture provide enough material to run reasonably intense EPR spectra. Computer-aided analysis of the EPR spectra of silica-bound nitroxides provided information on the sensitivity of the labeled silica monitoring different environments, e.g., to follow the path of particles in a mammalian cell culture. Upon contact of the particles with mesothelial cells, the mean distance among the labels at the silica surface decreased as a consequence of the release of oxidizing and/or radical moieties from the cells. |
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Authors:
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L Mollo; V Levresse; M F Ottaviani; S Ellouk-Achard; M C Jaurand; B Fubini |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Environmental health perspectives Volume: 105 Suppl 5 ISSN: 0091-6765 ISO Abbreviation: Environ. Health Perspect. Publication Date: 1997 Sep |
Date Detail:
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Created Date: 1998-01-30 Completed Date: 1998-01-30 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0330411 Medline TA: Environ Health Perspect Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1031-6 Citation Subset: IM |
Affiliation:
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Univeristà di Torino, Facoltà di Farmacia, Dipartimento di Chimica Inorganica, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cells, Cultured Electron Spin Resonance Spectroscopy Epithelial Cells / metabolism Epithelium / chemistry, metabolism Micelles Particle Size Pleura / cytology, metabolism Proteins / chemistry Rats Silicon Dioxide / chemistry* Spin Labels Surface Properties |
| Chemical | |
Reg. No./Substance:
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0/Micelles; 0/Proteins; 0/Spin Labels; 7631-86-9/Silicon Dioxide |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): Environ Health Perspect ISSN: 0091-6765 |
Article Information Download PDF  Print publication date: Month: 9 Year: 1997 Volume: 105 Issue: Suppl 5 First Page: 1031 Last Page: 1036 ID: 1470159 PubMed Id: 9400695 |
| Study of the stability of a paramagnetic label linked to mesoporous silica surface in contact with rat mesothelial cells in culture. | |
| L Mollo | |
| V Levresse | |
| M F Ottaviani | |
| S Ellouk-Achard | |
| M C Jaurand | |
| B Fubini | |
| Univerist? di Torino, Facolt? di Farmacia, Dipartimento di Chimica Inorganica, Italy. |
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