Document Detail

Study of stability of methotrexate in acidic solution spectrofluorimetric determination of methotrexate in pharmaceutical preparations through acid-catalyzed degradation reaction.
MedLine Citation:
PMID:  14565545     Owner:  NLM     Status:  MEDLINE    
Study of the degradation reaction of methotrexate (MTX) in acidic solution was carried out. Optimization of the experimental parameters of MTX acid hydrolysis was investigated. Spectrofluorimetric method for determination of MTX through measurement of its acid-degradation product, 4-amino-4-deoxy-10-methylpteroic acid (AMP), was developed. Stability of the standard solution of MTX prepared in sulfuric acid was discussed in the view of accelerated stability analysis. Two other comparative spectroflourimetric methods based on measuring the fluorescence intensities from either a condensation reaction with acetylacetone-formaldehyde (Hantzsch reaction) or a reaction with fluorescamine were also described. Beer's law validation, accuracy, precision, limits of detection, limits of quantification, and other aspects of analytical merit are presented in the text. The proposed methods were successfully applied for the analysis of MTX in pure drug and tablets dosage form. The sensitivity of the developed methods was favorable, so it was possible to be adopted for determination of MTX in plasma samples for routine use in high-dose MTX therapy.
Suzy M Sabry; M Abdel-Hady; M Elsayed; Osama T Fahmy; Hadir M Maher
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of pharmaceutical and biomedical analysis     Volume:  32     ISSN:  0731-7085     ISO Abbreviation:  J Pharm Biomed Anal     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-10-20     Completed Date:  2003-11-03     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8309336     Medline TA:  J Pharm Biomed Anal     Country:  England    
Other Details:
Languages:  eng     Pagination:  409-23     Citation Subset:  IM    
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, University of Alexandria, Alexandria, Egypt.
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MeSH Terms
Antimetabolites, Antineoplastic / chemistry*
Drug Stability
Fluorescamine / chemistry
Hydrogen-Ion Concentration
Indicators and Reagents
Methotrexate / chemistry*
Reference Standards
Spectrometry, Fluorescence
Reg. No./Substance:
0/Acids; 0/Antimetabolites, Antineoplastic; 0/Indicators and Reagents; 0/Solutions; 0/Tablets; 38183-12-9/Fluorescamine; 59-05-2/Methotrexate

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