| Study on the resonance light-scattering spectrum of anionic dye xylenol orange-cetyltrimethylammonium-nucleic acids system and determination of nucleic acids at nanogram levels. | |
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MedLine Citation:
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PMID: 15911414 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The interaction of xylenol orange (XO) and nucleic acids in the presence of cetyltrimethylammonium bromide (CTMAB) in aqueous solution has been studied by a resonance light-scattering (RLS) technique with a common spectrofluorometer. In hexamethylenetetramine (HMTA) buffer (pH7.30), XO and nucleic acids react with cetyltrimethylammonium bromide to form large particles of three-component complex, which results in strong enhanced RLS signals characterized by three peaks at 295.9, 335.5 and 542 nm, Mechanistic studies showed that the enhanced RLS stems from the aggregation of XO on DNA through the bridged and synergistic effect of CTMAB. With the enhanced RLS signals at the three wavelengths, the enhanced RLS intensity is proportional to the concentration of nucleic acids in an appropriate range. The lowest limit of determination was 5.31 ng ml(-1), three synthetic samples of yDNA were analyzed satisfactorily. |
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Authors:
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Xiaoming Chen; Changqun Cai; He 'an Luo; Guanghuo Zhang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Volume: 61 ISSN: 1386-1425 ISO Abbreviation: Spectrochim Acta A Mol Biomol Spectrosc Publication Date: 2005 Jul |
Date Detail:
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Created Date: 2005-05-24 Completed Date: 2005-10-31 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9602533 Medline TA: Spectrochim Acta A Mol Biomol Spectrosc Country: England |
Other Details:
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Languages: eng Pagination: 2215-20 Citation Subset: IM |
Affiliation:
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Institute of Chemistry, Xiangtan University, Xiangtan 411105, PR China. cxm@xtu.edu.cn |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cetrimonium Compounds
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chemistry* Coloring Agents / chemistry* Hydrogen-Ion Concentration Ions / chemistry Light Methenamine / chemistry Molecular Structure Nucleic Acids / analysis*, chemistry* Osmolar Concentration Scattering, Radiation Spectrum Analysis Viscosity Xylenes / chemistry*, radiation effects |
| Chemical | |
Reg. No./Substance:
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0/Cetrimonium Compounds; 0/Coloring Agents; 0/Ions; 0/Nucleic Acids; 0/Xylenes; 100-97-0/Methenamine; 1611-35-4/xylenol orange; 6899-10-1/cetrimonium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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