Document Detail


Study of the presence of the spores of Clostridium botulinum in honey in Brazil.
MedLine Citation:
PMID:  10397326     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The isolation of Clostridium botulinum from honey samples is described. Botulism is characterized as an intoxication provoked by ingestion of contaminated foods with this toxin. Infant botulism happens by the ingestion of spores of C. botulinum together with food that in special conditions of the intestinal tract, such as those present in babies of less than 1 year old, will allow the germination and colonization of the intestine with production and absorption of botulinic toxin. The samples were subjected to dilution and to a thermal shock and cultivated in modified CMM (Difco). Cultures were subjected to Gram smears and toxicity tests in mice. The toxic cultures were purified in RFCA (Oxoid) plates and incubated in anaerobic jars. Positive samples were typed using the mouse assay neutralization test. From the 85 honey samples analyzed, six were positive for C. botulinum (7.06%), and identified as producers of type A, B, and D toxins.
Authors:
R P Schocken-Iturrino; M C Carneiro; E Kato; J O Sorbara; O D Rossi; L E Gerbasi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEMS immunology and medical microbiology     Volume:  24     ISSN:  0928-8244     ISO Abbreviation:  FEMS Immunol. Med. Microbiol.     Publication Date:  1999 Jul 
Date Detail:
Created Date:  1999-08-26     Completed Date:  1999-08-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9315554     Medline TA:  FEMS Immunol Med Microbiol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  379-82     Citation Subset:  IM    
Affiliation:
Departamento de Patologia Veterinária, Universidade Estadual Paulista-UNESP, Faculdade de Ciencias Agrarias e Veterinárias de Jaboticabal, SP, Brazil. pablo@fcav.unesp.br
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MeSH Terms
Descriptor/Qualifier:
Animals
Brazil
Clostridium botulinum / isolation & purification*
Honey / microbiology*
Mice
Spores, Bacterial

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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