Document Detail

Study of the photodynamic effect on the A549 cell line by atomic force microscopy and the influence of green tea extract on the production of reactive oxygen species.
MedLine Citation:
PMID:  19723103     Owner:  NLM     Status:  MEDLINE    
We studied the morphology of the A549 cell line (human lung carcinoma cells) before and after photodynamic therapy (PDT) by atomic force microscopy. PDT was induced by an efficient light-emitting diode source with total light dose of 15 J cm(-2) in the presence of the sensitizer zinc-5,10,15,20-tetrakis(4-sulfonatophenyl)porphyrine. In the presence of molecular oxygen, light activation of the photosensitizer, which accumulates in cancer cells, leads to the local production of reactive oxygen species (ROS). This is one of several reasons leading to cell death, and in some cases we could observe signs of apoptosis. We detected the kinetics of ROS production to be dependent on the presence of green tea extract.
Katerina Tomankova; Hana Kolarova; Robert Bajgar; Dagmar Jirova; Kristina Kejlova; Jiri Mosinger
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Annals of the New York Academy of Sciences     Volume:  1171     ISSN:  1749-6632     ISO Abbreviation:  Ann. N. Y. Acad. Sci.     Publication Date:  2009 Aug 
Date Detail:
Created Date:  2009-09-02     Completed Date:  2009-09-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7506858     Medline TA:  Ann N Y Acad Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  549-58     Citation Subset:  IM    
Department of Medical Biophysics, Faculty of Medicine, Palacky University in Olomouc, Olomouc, Czech Republic.
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MeSH Terms
Apoptosis / drug effects
Cell Line, Tumor
Cell Survival / drug effects
Dose-Response Relationship, Drug
Lung Neoplasms / metabolism,  pathology
Metalloporphyrins / pharmacology
Microscopy, Atomic Force / methods*
Oxygen / metabolism,  pharmacology
Photochemotherapy / methods
Photosensitizing Agents / pharmacology
Plant Extracts / pharmacology*
Reactive Oxygen Species / metabolism*
Tea / chemistry*
Reg. No./Substance:
0/Metalloporphyrins; 0/Photosensitizing Agents; 0/Plant Extracts; 0/Reactive Oxygen Species; 0/Tea; 0/zinc-tetrakis(p-sulfonatophenyl)porphyrin; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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