Document Detail


Study on ovine abortion associated with Toxoplasma gondii in affected herds of Khorasan Razavi Province, Iran based on PCR detection of fetal brains and maternal serology.
MedLine Citation:
PMID:  24650931     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
SUMMARYToxoplasma gondii, an obligatory intracellular protozoan parasite, is one of the causative agents of ovine abortion, as reported in many countries. Different techniques are being used to detect this pathogen in infected ovine fetuses. One of the most sensitive and specific diagnostic techniques is Nested-PCR amplification of the B1 target gene of the organism. In total, 200 brain samples of aborted ovine fetuses and maternal sera submitted from different parts of Khorasan Razavi province, Iran were investigated to track the role of Toxoplasma gondii in ovine abortion by a slightly modified Nested-PCR and IFAT assays, respectively. Among all samples, 27 (13·5%) were PCR-positive and 31 (15·5%) were IFAT-positive and the Toxoplasma-induced abortion prevalence calculated was 8·8% to18·2% with 95% confidence interval. Results show that high levels of congenital transmission may occur in 27/31(87%) of pregnancies with an excellent logical agreement (ĸ=0·9) between 2 different tests. According to the results of this study, the Nested-PCR employed in this investigation could be recommended as an applied routine test for the routine examination and confirmation of Toxoplasma gondii-induced ovine abortion.
Authors:
M Rassouli; G R Razmi; M R Bassami; A R Movassaghi; M Azizzadeh
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Parasitology     Volume:  138     ISSN:  1469-8161     ISO Abbreviation:  Parasitology     Publication Date:  2011 May 
Date Detail:
Created Date:  2014-03-21     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0401121     Medline TA:  Parasitology     Country:  England    
Other Details:
Languages:  eng     Pagination:  691-7     Citation Subset:  IM    
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