Document Detail


Study on an on-line molecularly imprinted solid-phase extraction coupled to high-performance liquid chromatography for separation and determination of trace estrone in environment.
MedLine Citation:
PMID:  19104786     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Estrone is one of the important potential endocrine-disrupting compounds, and the sensitive and reliable analytical methods for the determination of estrone are required for the assurance of human health. In this paper, using estrone as template molecule, 3-aminopropyltriethoxysilane as function monomer, and tetraethoxysilicane as cross-linker, a highly selective molecularly imprinted microsphere was synthesized by surface molecular imprinting technique combined with a sol-gel process. The imprinted material was characterized by the Fourier transform infrared and static adsorption experiments, and the results showed that it exhibited good recognition and selective ability for estrone. A novel method for separation and determination of trace estrone in environmental sample was developed using on-line molecularly imprinted solid-phase extraction coupled to high-performance liquid chromatography. With a sample loading flow rate of 2.6 mL min(-1) for a 9.6-min extraction, the enrichment factor obtained by the slopes of the linear portion in comparison with the direct injection of 10 microL standard sample solution was 1,045. The detection limit (S/N = 3) was 5.7 ng L(-1), and the relative standard deviations for nine replicate extractions of 5.0 microg L(-1) estrone was less than 10.0%. This method was evaluated for quantitative determination of estrone in well and lake water samples spiked at two levels (0.5 and 1.0 microg L(-1)) with recoveries ranging from 86% to 95%.
Authors:
Zhixiang Xu; Shuang Chen; Wei Huang; Guozhen Fang; Hua Pingzhu; Shuo Wang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-12-24
Journal Detail:
Title:  Analytical and bioanalytical chemistry     Volume:  393     ISSN:  1618-2650     ISO Abbreviation:  Anal Bioanal Chem     Publication Date:  2009 Feb 
Date Detail:
Created Date:  2009-02-09     Completed Date:  2009-04-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101134327     Medline TA:  Anal Bioanal Chem     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1273-9     Citation Subset:  IM    
Affiliation:
Key Laboratory of Food Nutrition and Safety, Ministry of Education of China, Tianjin University of Science and Technology, Tianjin, 300457, China.
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MeSH Terms
Descriptor/Qualifier:
Adsorption
Chromatography, High Pressure Liquid / methods*
Environmental Pollutants / analysis*
Estrone / analysis*
Polymers / chemistry
Reproducibility of Results
Sensitivity and Specificity
Spectrophotometry, Ultraviolet
Spectroscopy, Fourier Transform Infrared
Chemical
Reg. No./Substance:
0/Environmental Pollutants; 0/Polymers; 53-16-7/Estrone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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