Document Detail


Study of the interference problems of dioxin-like chemicals with the bio-analytical method CALUX.
MedLine Citation:
PMID:  18969556     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The reporter gene expression method CALUX has proven to be a very valuable screening technique for assessing toxic equivalents of dioxins and dioxin-like compounds, because it detects all AhR ligands in a variety of sample matrices. However, the exact meaning of the CALUX response is difficult to evaluate for complex mixtures mainly since not all AhR ligands are known and since antagonistic or synergistic effects occurs. In this paper, non-additive effects on the CALUX response of dioxins were investigated for a limited number of dioxin-like compounds in concentration ranges that are 10(2)-10(8) times higher than that of 2,3,7,8-TCDD. Antagonistic effects are detected for three Aroclors (1242, 1254, 1260), Halowax 1014 (PCN), HCB and PBB 169. The ratios, Aroclor/dioxin, Halowax/dioxin and HCB/dioxin, needed to observe an antagonistic effect are 10000, 5000 and 50000, respectively. No significant deviation from additivity was observed for Aroclor 5442 (PCT) and PBB 77 in the concentration range investigated. Two clean-up procedures have also been tested: in some cases the non-additive effects disappeared or were strongly reduced. Using only an acidic silica column, the classical dioxin-like compounds investigated here (PCB, PCT, PBB, PCN, HCB) as well as the dioxins are collected and analyzed altogether in one fraction. Consequently, no major alteration of the non-additive effects is expected. An acidic silica column combined with an activated carbon column allows the separation of PCDD/F and dioxin-like PCB in two different fractions, PBB 169 is completely eluted in the dioxin fraction and PBB 77 is distributed between the PCB and dioxin fraction. HCB is completely separated from the PCDD/F fraction.
Authors:
Carmen Schroijen; Isabelle Windal; Leo Goeyens; Willy Baeyens
Related Documents :
11993636 - Patterns of pcbs and pcdd/pcdfs in chicken and pork fat following a belgian food contam...
9601926 - Hepatic enzyme induction and acute endocrine effects of 2,3,3',4',6-pentachlorobiphenyl...
20145996 - Dietary exposure of three passerine species to pcdd/dfs from the chippewa, tittabawasse...
16343596 - Preliminary data on polybrominated diphenyl ethers (pbdes) in farmed fish tissues (salm...
8335036 - Pharmacokinetic studies in healthy volunteers in the context of in vitro/in vivo correl...
11975366 - Nuclear and isotopic techniques for addressing nutritional problems, with special refer...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Talanta     Volume:  63     ISSN:  1873-3573     ISO Abbreviation:  Talanta     Publication Date:  2004 Aug 
Date Detail:
Created Date:  2008-10-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984816R     Medline TA:  Talanta     Country:  England    
Other Details:
Languages:  eng     Pagination:  1261-8     Citation Subset:  -    
Affiliation:
Department of Analytical and Environmental Chemistry, Free University of Brussels, Pleinlaan 2, 1050 Brussels, Belgium.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Importance of REP values when comparing the CALUX bioassay results with chemoanalyses results Exampl...
Next Document:  Importance of clean-up for comparison of TEQ-values obtained by CALUX and chemo-analysis.