Document Detail


Study on the interaction between silver nanoparticles and nucleic acids in the presence of cetyltrimethylammonium bromide and its analytical application.
MedLine Citation:
PMID:  18371671     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A novel method is proposed in this paper, that is the silver nanoparticle (nanoAg)-cetyltrimethylammonium bromide (CTMAB) is used as the probe of resonance light scattering (RLS) for the determination of nucleic acids. Under optimum conditions, there are linear relationships between the quenching extent of RLS and the concentration of nucleic acids in the range of 4.0x10(-9)-2.0x10(-6)gmL(-1) for fish sperm DNA (fsDNA), 7.0x10(-9)-1.8x10(-6)gmL(-1) for calf thymus DNA (ctDNA) and 6.0x10(-9)-1.0x10(-6)gmL(-1) for yeast RNA (yRNA). The detection limits (S/N=3) of fsDNA, ctDNA and yRNA are 2.7x10(-10)gmL(-1), 4.8x10(-10)gmL(-1) and 7.2x10(-10)gmL(-1), respectively. The studies indicate that there are interactions among nanoAg, CTMAB and fsDNA through electrostatic and chemical affinity, and the nanoAg-CTMAB complex can induce the structural change of base stacking and helicity of fsDNA.
Authors:
Jinhua Zheng; Xia Wu; Minqin Wang; Dehuan Ran; Wei Xu; Jinhe Yang
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-06-19
Journal Detail:
Title:  Talanta     Volume:  74     ISSN:  1873-3573     ISO Abbreviation:  Talanta     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2008-03-28     Completed Date:  2008-08-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  2984816R     Medline TA:  Talanta     Country:  England    
Other Details:
Languages:  eng     Pagination:  526-32     Citation Subset:  IM    
Affiliation:
Key Laboratory of Colloid and Interface Chemistry (Shandong University), Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, Shandong, PR China.
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MeSH Terms
Descriptor/Qualifier:
Calibration
Cetrimonium Compounds / chemistry*
Hydrogen-Ion Concentration
Metal Nanoparticles*
Nucleic Acids / chemistry*
Sensitivity and Specificity
Silver / chemistry*
Chemical
Reg. No./Substance:
0/Cetrimonium Compounds; 0/Nucleic Acids; 6899-10-1/cetrimonium; 7440-22-4/Silver

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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