| Study on the interaction between nucleic acid and Eu3+-oxolinic acid and the determination of nucleic acid using the resonance light scattering technique. | |
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MedLine Citation:
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PMID: 15951231 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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At pH 9.75, the resonance light scattering (RLS) intensity of OA-Eu3+ system is greatly enhanced by nucleic acid. Based on this phenomenon, a new quantitative method for nucleic acid in aqueous solution has been developed. Under the optimum condition, the enhanced RLS is proportional to the concentration of nucleic acid in the range of 1.0x10(-9) to 1.0x10(-6)g/ml for herring sperm DNA, 8.0x10(-10) to 1.0x10(-6) g/ml for calf thymus DNA and 1.0x10(-9) to 1.0x10(-6) g/ml for yeast RNA, and their detection limits are 0.020, 0.011 and 0.010 ng/ml, respectively. Synthetic samples and actual samples were satisfactorily determined. In addition, the interaction mechanism between nucleic acid and OA-Eu3+ is also investigated. |
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Authors:
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Xia Wu; Shuna Sun; Jinghe Yang; Minqin Wang; Liyan Liu; Changying Guo |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-06-13 |
Journal Detail:
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Title: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy Volume: 62 ISSN: 1386-1425 ISO Abbreviation: Spectrochim Acta A Mol Biomol Spectrosc Publication Date: 2005 Dec |
Date Detail:
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Created Date: 2005-11-23 Completed Date: 2006-03-28 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9602533 Medline TA: Spectrochim Acta A Mol Biomol Spectrosc Country: England |
Other Details:
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Languages: eng Pagination: 896-901 Citation Subset: IM |
Affiliation:
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Key Laboratory of Colloid and Interface Chemistry, Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan 250100, Shandong, PR China. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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DNA
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analysis Europium / chemistry* Hydrogen-Ion Concentration Molecular Probes Nucleic Acids / analysis*, chemistry*, ultrastructure Oxolinic Acid / chemistry* Phosphates / pharmacology Scattering, Radiation Spectrum Analysis Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Molecular Probes; 0/Nucleic Acids; 0/Phosphates; 14698-29-4/Oxolinic Acid; 7440-53-1/Europium; 9007-49-2/DNA; 91080-16-9/calf thymus DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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