Document Detail

Study of the in vitro maturation of mouse oocytes induced by microinjection of maturation promoting factor (MPF).
MedLine Citation:
PMID:  10754786     Owner:  NLM     Status:  MEDLINE    
PURPOSE: Maturation promoting factor (MPF) acts at the resumption of meiosis and nonspecifically throughout the animal species. There exists a considerable body of literature on MPF, but little work has been done to study the induction of maturation of mammalian oocytes by microinjection of extracted MPF.
METHODS: Immature (GV-stage) mouse oocytes were microinjected MPF extracted from matured Xenopus eggs in the presence of dbcAMP.
RESULTS: The rate of germinal vesicle, breakdown (GVBD) induced at 24 hr after MPF injection was significantly higher (90.5%) than that of the control (2.2%), which was injected with HTF medium containing dbcAMP (P < 0.0001). The rate of extrusion of the first polar body at 24 hr after MPF injection was significantly higher (84.1%) than that of the same control (1.1%) (P < 0.0001).
CONCLUSIONS: From these results, it is concluded that the maturation of mammalian oocytes can be induced by the microinjection of MPF extracted from other species.
H Nakano; H Kubo
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of assisted reproduction and genetics     Volume:  17     ISSN:  1058-0468     ISO Abbreviation:  J. Assist. Reprod. Genet.     Publication Date:  2000 Jan 
Date Detail:
Created Date:  2000-05-25     Completed Date:  2000-05-25     Revised Date:  2013-03-26    
Medline Journal Info:
Nlm Unique ID:  9206495     Medline TA:  J Assist Reprod Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  67-73     Citation Subset:  IM    
First Department of Obstetrics and Gynecology, Toho University School of Medicine, Tokyo, Japan.
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MeSH Terms
Cells, Cultured
Cyclic AMP / pharmacology
Maturation-Promoting Factor / isolation & purification,  pharmacology*
Mice, Inbred ICR
Oocytes / cytology,  drug effects*,  physiology*
Xenopus laevis
Reg. No./Substance:
60-92-4/Cyclic AMP; EC Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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