Document Detail


Study of force induced melting of dsDNA as a function of length and conformation.
MedLine Citation:
PMID:  21386589     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
We measure the constant force required to melt double-stranded (ds) DNA as a function of length for lengths from 12 to 100 000 base pairs, where the force is applied to the 3'3' or 5'5' ends of the dsDNA. Molecules with 32 base pairs or fewer melt before overstretching. For these short molecules, the melting force is independent of the ends to which the force is applied and the shear force as a function of length is well described by de Gennes theory with a de Gennes length of less than 10 bp. Molecules with lengths of 500 base pairs or more overstretch before melting. For these long molecules, the melting force depends on the ends to which the force is applied. The melting force as a function of length increases even when the length exceeds 1000 bp, where the length dependence is inconsistent with de Gennes theory. Finally, we expand de Gennes melting theory to 3'5' pulling and compare the predictions with experimental results.
Authors:
Claudia Danilowicz; Kristi Hatch; Alyson Conover; Theodore Ducas; Ruwan Gunaratne; Vincent Coljee; Mara Prentiss
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Publication Detail:
Type:  Journal Article     Date:  2010-09-30
Journal Detail:
Title:  Journal of physics. Condensed matter : an Institute of Physics journal     Volume:  22     ISSN:  0953-8984     ISO Abbreviation:  J Phys Condens Matter     Publication Date:  2010 Oct 
Date Detail:
Created Date:  2011-03-09     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101165248     Medline TA:  J Phys Condens Matter     Country:  England    
Other Details:
Languages:  eng     Pagination:  414106     Citation Subset:  IM    
Affiliation:
Department of Physics, Harvard University, Cambridge, MA 02138, USA.
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