| Study on the effect of eicosapentaenoic acid on phospholipids composition in membrane microdomains of tight junctions of epithelial cells by liquid chromatography/electrospray mass spectrometry. | |
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MedLine Citation:
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PMID: 18280689 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Tight junctions of epithelial cells determine epithelial membrane integrity and play an important role in selective paracellular permeability to ions and macromolecules. In this work, we investigated the effect of one of n-3 series polyunsaturated fatty acids, eicosapentaenoic acid (EPA) on the phospholipid composition of membrane microdomains of tight junctions. After treated by EPA, membrane microdomains of tight junctions were isolated by discontinuous sucrose density gradient ultracentrifugation, and raft phospholipids were extracted. The PE, PI, PS, PC and SM were separated and determined by high-performance liquid chromatography/quadrupole-linear ion trap mass spectrometry (HPLC Qtrap-MS), and were further identified by HPLC-MS/MS. It was found that EPA altered the fatty acyl substitution of phospholipids that constituted membrane microdomains of tight junctions by enriching the unsaturated fatty acyl chains of the phospholipids. It provides a new visual angle to explaining the intracellular mechanism involved in n-3 polyunsaturated fatty acids (PUFAs) modulation of intestinal tight junction barrier. |
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Authors:
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Sumin Zhao; Lewen Jia; Peng Gao; Qiurong Li; Xin Lu; Jieshou Li; Guowang Xu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-01-09 |
Journal Detail:
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Title: Journal of pharmaceutical and biomedical analysis Volume: 47 ISSN: 0731-7085 ISO Abbreviation: J Pharm Biomed Anal Publication Date: 2008 Jun |
Date Detail:
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Created Date: 2008-05-12 Completed Date: 2008-07-29 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8309336 Medline TA: J Pharm Biomed Anal Country: England |
Other Details:
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Languages: eng Pagination: 343-50 Citation Subset: IM |
Affiliation:
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National Chromatographic R&A Center, Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Line Chromatography, High Pressure Liquid Eicosapentaenoic Acid / pharmacology* Epithelial Cells / metabolism*, physiology Humans Membrane Microdomains* / chemistry, drug effects, physiology Phospholipids / analysis*, chemistry Spectrometry, Mass, Electrospray Ionization Tight Junctions / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Phospholipids; 1553-41-9/Eicosapentaenoic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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