Document Detail


Study of direct immunofluorescence, immunofluorescence mapping and light microscopy in porphyria cutanea tarda.
MedLine Citation:
PMID:  21308306     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Even though porphyria cutanea tarda is the most frequent type of porphyria, there are few studies about its cutaneous physiopathology.
OBJECTIVE: To evaluate skin changes in porphyria cutanea tarda using light microscopy and direct immunofluorescence before and after treatment with chloroquine. To perform antigen immunomapping of bullae to study their level of cleavage.
METHODS: Light microscopy and direct immunofluorescence of 28 patients are reported in three different phases: 23 patients with active porphyria before treatment (Phase A), 7 patients with clinical remission during treatment (Phase B), and 8 patients with biochemical remission (Phase C). Immunomapping was performed on 7 patients.
RESULTS: In active porphyria, direct immunofluorescence showed homogenous and intense fluorescence on the inside and on the walls of blood vessels as well as in the dermal-epidermal junction. In clinical remission (Phase B) and biochemical remission (Phase C), the deposit of immunoglobulins was maintained, but the deposit of complement was reduced in most cases. Immunomapping revealed no standard cleavage plane.
CONCLUSION: No correlation was observed between clinical response and immunoglobulin deposits. The reduction of complement favors the hypothesis that activation of the complement cascade represents an additional pathway that leads to endothelial damage.
Authors:
Fátima Mendonça Jorge Vieira; Valéria Aoki; Zilda Najjar Prado de Oliveira; José Eduardo Costa Martins
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Anais brasileiros de dermatologia     Volume:  85     ISSN:  1806-4841     ISO Abbreviation:  An Bras Dermatol     Publication Date:    2010 Nov-Dec
Date Detail:
Created Date:  2011-02-10     Completed Date:  2011-10-24     Revised Date:  2012-08-20    
Medline Journal Info:
Nlm Unique ID:  0067662     Medline TA:  An Bras Dermatol     Country:  Brazil    
Other Details:
Languages:  eng; por     Pagination:  827-37     Citation Subset:  IM    
Affiliation:
Faculty of Medicine, University of Sao Paulo, Sao Paulo, SP, Brazil. fmjvieira@hotmail.com
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Adult
Aged
Antibodies, Monoclonal / immunology
Antigens / immunology
Chloroquine / therapeutic use
Cross-Sectional Studies
Dermatologic Agents / therapeutic use
Female
Fluorescent Antibody Technique, Direct
Humans
Male
Microscopy / methods
Middle Aged
Porphyria Cutanea Tarda / drug therapy,  immunology,  physiopathology*
Young Adult
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens; 0/Dermatologic Agents; 54-05-7/Chloroquine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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