Document Detail


Study of demembranated, reactivated human spermatozoa with decondensed nuclei.
MedLine Citation:
PMID:  10589509     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Reactivated movement of the axonemes in demembranated spermatozoa with decondensed nuclei allows decondensation to be monitored in vitro with minimal disruption, and provides access to the nucleus for ultrastructural investigation and experimental manipulation. In the present study, fresh liquefied semen samples with sperm concentrations > or = 13 x 10(6)/ml were diluted 1:10 with a demembranating solution containing 0.01-0.022% Triton X-100. Inter-sample variation in the concentration of Triton X-100 required to permeabilize the sperm membrane was observed as judged by the ability of the spermatozoa to be reactivated by ATP but not by an ATP-free control solution, with the extent of demembranation being checked by transmission electron microscopy. After exposure to DTT and heparin, coordinated and sometimes progressive movement of partially decondensed spermatozoa occurred in a reactivating solution. Unlike ram, human sperm heads required decondensation with heparin. An unusual ultrastructural feature of the decondensing human sperm nuclei, not previously reported, was the appearance of dense globular material extruding from the nucleus. Enzymatic treatment of the sections with protease but not with deoxyribonuclease removed this material, which was presumably protamine.
Authors:
H Bezanehtak; M A Swan
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Journal of experimental zoology     Volume:  284     ISSN:  0022-104X     ISO Abbreviation:  J. Exp. Zool.     Publication Date:  1999 Dec 
Date Detail:
Created Date:  1999-12-22     Completed Date:  1999-12-22     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0375365     Medline TA:  J Exp Zool     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  789-97     Citation Subset:  IM    
Affiliation:
Department of Anatomy and Histology, University of Sydney, NSW, Australia.
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MeSH Terms
Descriptor/Qualifier:
Adenosine Triphosphate / pharmacology
Cell Nucleus / drug effects,  physiology*,  ultrastructure
Chromatin / drug effects,  ultrastructure
Dithiothreitol / pharmacology
Heparin / pharmacology
Humans
Intracellular Membranes* / drug effects,  ultrastructure
Male
Octoxynol / pharmacology
Sperm Count
Sperm Head / drug effects,  ultrastructure
Sperm Motility / drug effects,  physiology*
Spermatozoa / drug effects,  physiology*,  ultrastructure
Chemical
Reg. No./Substance:
0/Chromatin; 3483-12-3/Dithiothreitol; 56-65-5/Adenosine Triphosphate; 9002-93-1/Octoxynol; 9005-49-6/Heparin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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