Document Detail

Study of cytotoxic effect of photodynamically and sonodynamically activated sensitizers in vitro.
MedLine Citation:
PMID:  19595758     Owner:  NLM     Status:  MEDLINE    
High resolution imaging of biological structures and their changes induced by different agents such as drugs are commonly performed by confocal and electron microscopy. The past decade has witnessed an emersion of the atomic force microscopy (AFM) from solid-state physics into cell biology and even medical applications. For these reasons, we used this relatively new microscopic technique to study the morphology of cell lines. We imaged the cells by atomic force microscopy before and after the photodynamic therapy (PDT) using the photosensitizer ClAlPcS(2). We also compared the impact of the photosensitizer in combination with silymarin antioxidant on cancer and non-cancer cell lines by measuring the kinetic production of reactive oxygen species (ROS). PDT was induced by LED source with total irradiation dose of 15 J cm(-2) and SDT was induced by therapeutic ultrasound with frequency of 1 MHz, intensity 2 W cm(-2) and time of exposition 10 min. The results show ROS kinetic production within the cells during PDT, sonodynamic therapy (SDT) and modification of morphological features investigated by AFM. The combination of a sensitizer and the specific light source can lead to the loss of surface rigidity and eventually to dramatic changes of the cell shape, which we can study by AFM.
K Tomankova; H Kolarova; P Kolar; K Kejlova; D Jirova
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-07-10
Journal Detail:
Title:  Toxicology in vitro : an international journal published in association with BIBRA     Volume:  23     ISSN:  1879-3177     ISO Abbreviation:  Toxicol In Vitro     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2009-11-13     Completed Date:  2010-01-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8712158     Medline TA:  Toxicol In Vitro     Country:  England    
Other Details:
Languages:  eng     Pagination:  1465-71     Citation Subset:  IM    
Department of Medical Biophysics, Faculty of Medicine, Palacky University in Olomouc, Hnevotinska 3, 775 15 Olomouc, Czech Republic.
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MeSH Terms
Apoptosis / drug effects
Dose-Response Relationship, Drug
Indoles / pharmacology*
Microscopy, Atomic Force
NIH 3T3 Cells
Organometallic Compounds / pharmacology*
Photosensitizing Agents / pharmacology*
Reactive Oxygen Species / metabolism
Ultrasonic Therapy*
Reg. No./Substance:
0/Indoles; 0/Organometallic Compounds; 0/Photosensitizing Agents; 0/Reactive Oxygen Species; 68637-19-4/aluminum phthalocyanine disulfonate

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