Document Detail


Study of the cytolethal distending toxin-induced cell cycle arrest in HeLa cells: involvement of the CDC25 phosphatase.
MedLine Citation:
PMID:  10854069     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
HeLa cells exposed to Escherichia coli cytolethal distending toxins (CDT) arrest their cell cycle at the G2/M transition. We have shown previously that in these cells the CDK1/cyclin B complex is inactive and can be reactivated in vitro using recombinant CDC25 phosphatase. Here we have investigated in vivo the effects of CDC25 on this cell cycle checkpoint. We report that overexpression of CDC25B or CDC25C overrides an established CDT-induced G2 cell cycle arrest and leads the cells to accumulate in an abnormal mitotic stage with condensed chromatin and high CDK1 activity. This effect can be counteracted by coexpression of the WEE1 kinase. In contrast, overexpression of CDC25B or C prior to CDT treatment prevents G2 arrest and allows most of the cells to progress through mitosis with only a low percentage of cells arrested in abnormal mitosis. The implications of these results on the biochemical nature of the CDT-induced cell cycle arrest are discussed.
Authors:
N Escalas; N Davezac; J De Rycke; V Baldin; R Mazars; B Ducommun
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental cell research     Volume:  257     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  2000 May 
Date Detail:
Created Date:  2000-07-06     Completed Date:  2000-07-06     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  206-12     Citation Subset:  IM    
Affiliation:
LBCMCP-CNRS, Université Paul Sabatier, Toulouse, France.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Toxins / toxicity*
Cell Cycle / drug effects*,  physiology*
Cell Cycle Proteins*
Escherichia coli
Hela Cells
Humans
Nuclear Proteins*
Protein-Tyrosine Kinases / physiology
Signal Transduction / drug effects,  physiology
cdc25 Phosphatases / physiology*
Chemical
Reg. No./Substance:
0/Bacterial Toxins; 0/Cell Cycle Proteins; 0/Nuclear Proteins; 0/cytolethal distending toxin; EC 2.7.10.1/Protein-Tyrosine Kinases; EC 2.7.10.2/WEE1 protein, human; EC 3.1.3.48/cdc25 Phosphatases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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