Document Detail

Study of cyclic adenosine monophosphate microdomains in cells.
MedLine Citation:
PMID:  15988051     Owner:  NLM     Status:  MEDLINE    
Cyclic adenosine monophosphate (cAMP) controls the physiological response to many diverse extracellular stimuli. To maintain signal specificity, cAMP-mediated signaling is finely tuned by means of a complex array of proteins that control the spatial and temporal dynamics of the second messenger within the cell. To unravel the way a cell encodes cAMP signals, new biosensors have recently been introduced that allow imaging of the second messenger in living cells with high spatial resolution. The more recent generation of such biosensors exploits the phenomenon of fluorescence resonance energy transfer between the green fluorescent protein- tagged subunits of a chimeric protein kinase A, as the way to visualize and measure the dynamic fluctuations of cAMP. This chapter describes the molecular basis on which such a genetically encoded cAMP sensor relies and the tools and methods required to perform cAMP measurements in living samples.
Marco Mongillo; Anna Terrin; Sandrine Evellin; Valentina Lissandron; Manuela Zaccolo
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  307     ISSN:  1064-3745     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2005  
Date Detail:
Created Date:  2005-06-30     Completed Date:  2005-09-15     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1-13     Citation Subset:  IM    
Dulbecco Telethon Institute and Venetian Institute of Molecular Medicine, Padova, Italy.
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MeSH Terms
Biosensing Techniques* / methods
COS Cells
Cyclic AMP / analysis*,  metabolism
Cyclic AMP-Dependent Protein Kinases / analysis,  genetics
Cytoplasm / chemistry*,  metabolism
Fluorescence Resonance Energy Transfer* / methods
Green Fluorescent Proteins / analysis,  genetics
Reg. No./Substance:
147336-22-9/Green Fluorescent Proteins; 60-92-4/Cyclic AMP; EC AMP-Dependent Protein Kinases

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