| Study of the cryotolerance of Lactobacillus acidophilus: effect of culture and freezing conditions on the viability and cellular protein levels. | |
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MedLine Citation:
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PMID: 11020044 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Slow cooling rate and pre-freezing stress brings about a high increase in the cell resistance and preservation of their physiological characteristics. A brutal decrease in temperature (from 37 degrees C to - 80 degrees C) causes a considerable loss of cell viability, in contrast a slow one preserves a survival rate of 75%. Pre-incubation of cells at low temperature (22 degrees C) during 6 h led to the development of cryotolerance indicated by an enhanced capacity to survive after a freezing treatment of 24 h at - 80 degrees C. Exposure of the cells to low pH (5.5) caused a large decrease in cell resistance but did not lead to any significant decrease of survival rate after freezing treatment. However, an increase of 15 +/- 3% in protein level compared to cells cultivated at regulated pH was observed. |
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Authors:
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L Bâati; C Fabre-Gea; D Auriol; P J Blanc |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of food microbiology Volume: 59 ISSN: 0168-1605 ISO Abbreviation: Int. J. Food Microbiol. Publication Date: 2000 Sep |
Date Detail:
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Created Date: 2000-10-26 Completed Date: 2000-10-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8412849 Medline TA: Int J Food Microbiol Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 241-7 Citation Subset: IM |
Affiliation:
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Centre de Bioingénierie Gilbert Durand, Laboratoire Biotechnologies-Bioprocédés, UMR CNRS 5504, LAA INRA 792, 135, Toulouse, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Proteins
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analysis* Freezing Hydrogen-Ion Concentration Lactobacillus acidophilus / chemistry, growth & development* |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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