Document Detail

Study of the cryotolerance of Lactobacillus acidophilus: effect of culture and freezing conditions on the viability and cellular protein levels.
MedLine Citation:
PMID:  11020044     Owner:  NLM     Status:  MEDLINE    
Slow cooling rate and pre-freezing stress brings about a high increase in the cell resistance and preservation of their physiological characteristics. A brutal decrease in temperature (from 37 degrees C to - 80 degrees C) causes a considerable loss of cell viability, in contrast a slow one preserves a survival rate of 75%. Pre-incubation of cells at low temperature (22 degrees C) during 6 h led to the development of cryotolerance indicated by an enhanced capacity to survive after a freezing treatment of 24 h at - 80 degrees C. Exposure of the cells to low pH (5.5) caused a large decrease in cell resistance but did not lead to any significant decrease of survival rate after freezing treatment. However, an increase of 15 +/- 3% in protein level compared to cells cultivated at regulated pH was observed.
L Bâati; C Fabre-Gea; D Auriol; P J Blanc
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of food microbiology     Volume:  59     ISSN:  0168-1605     ISO Abbreviation:  Int. J. Food Microbiol.     Publication Date:  2000 Sep 
Date Detail:
Created Date:  2000-10-26     Completed Date:  2000-10-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8412849     Medline TA:  Int J Food Microbiol     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  241-7     Citation Subset:  IM    
Centre de Bioingénierie Gilbert Durand, Laboratoire Biotechnologies-Bioprocédés, UMR CNRS 5504, LAA INRA 792, 135, Toulouse, France.
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MeSH Terms
Bacterial Proteins / analysis*
Hydrogen-Ion Concentration
Lactobacillus acidophilus / chemistry,  growth & development*
Reg. No./Substance:
0/Bacterial Proteins

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