| Study of catecholamines in patient urine samples by capillary electrophoresis. | |
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MedLine Citation:
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PMID: 10486762 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Capillary zone electrophoresis with photodiode array detection at 220 nm was used for analysis of catechol compounds in human urine. The method was optimized with reference compounds 3,4-dihydroxybenzylamine, adrenaline, noradrenaline, normetanephrine, dopamine, dopac (homogensitic acid), methanephrine, vanillyl-mandelic acid, 5-hydroxyindoleacetic acid (5-HIAA), homovanillic acid and 3-methoxytyramic acid at pH 4.0 and 8.0 for their electrophoretic separation. The UV spectra of the catechols were detected at a concentration of 20 microM. Repeatability of the method calculated using the absolute migration times of the catechols was below 1.5% and using the peak areas below 5%. The patient samples were hydrolyzed by 0.5 M acid or base solutions. In the studies, a few patient samples were analyzed using 3,4-dihydroxybenzylamine as an internal standard. In the hydrolysis steps needed for their detection in urine, all the other catecholamines, except 5-HIAA, did not decompose to detectable species at 220 or 254 nm. The concentrations of the catecholamines observed in real samples were at nM levels. |
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Authors:
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H Sirén; U Karjalainen |
Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Journal of chromatography. A Volume: 853 ISSN: 0021-9673 ISO Abbreviation: J Chromatogr A Publication Date: 1999 Aug |
Date Detail:
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Created Date: 1999-10-26 Completed Date: 1999-10-26 Revised Date: 2009-01-15 |
Medline Journal Info:
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Nlm Unique ID: 9318488 Medline TA: J Chromatogr A Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 527-33 Citation Subset: IM |
Affiliation:
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Academy of Finland, Espoo, Finland. heli.siren@vtt.fi |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Catecholamines
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analysis,
metabolism,
urine* Chromatography, High Pressure Liquid / methods Electrophoresis, Capillary / methods* Humans Hydrolysis Spectrophotometry, Ultraviolet |
| Chemical | |
Reg. No./Substance:
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0/Catecholamines |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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