| Study of binding of 12(S)-hydroxy-5(Z),8(Z),10(E), 14(Z)-eicosatetraenoic acid to bovine serum albumin using dynamic surface tension measurements. | |
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MedLine Citation:
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PMID: 10585225 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In a recent paper,(1) we demonstrated that molecular interactions between biopolymers and other smaller molecules can be detected by means of dynamic surface tension measurements. In the present paper, we demonstrate that the same methodology can be employed for investigating dose effects and specificity of molecular interactions. Three similar lipids were chosen for this study: 12(S)-hydroxy-5(Z), 8(Z),10(E),14(Z)-eicosatetraenoic acid (12(S)-HETE-free acid), methyl 12(S)-hydroxy-5(Z),8(Z),10(E),14(Z)-eicosatetraenoate (12(S)-HETE-methyl ester), and 5(Z),8(Z),11(Z), 14(Z)-eicosatetraenoic acid (arachidonic acid-free acid). These substances were added to a fatty acid free bovine serum albumin (BSA) aqueous solution at different lipid concentrations. The characteristic tension response indicates that molecular interactions between 12(S)-HETE-free acid and BSA exist. The detected interactions are concentration dependent: at a molecular ratio of lipid to protein of 1:1, the binding of 12(S)-HETE-free acid to BSA is hydrophobic in nature; at the molecular ratio of lipid to protein of 10:1, a secondary binding occurs and is hydrophilic in nature. Similar molecular interactions were not detected between 12(S)-HETE-methyl ester or arachidonic acid-free acid and BSA, indicating that the interactions between 12(S)-HETE-free acid and BSA are specific. As an independent means, surface elasticity is used to probe the molecular interactions at the interface. In the case of 12(S)-HETE-free acid but not its methyl ester or arachidonic acid, distinct higher surface elasticities were observed at lipid concentrations in excess of a molecular ratio of lipid to protein of 1:1. This finding reinforces the above stipulations. |
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Authors:
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P Chen; Z Policova; C R Pace-Asciak; A W Neumann |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of pharmaceutical sciences Volume: 88 ISSN: 0022-3549 ISO Abbreviation: J Pharm Sci Publication Date: 1999 Dec |
Date Detail:
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Created Date: 2000-02-01 Completed Date: 2000-02-01 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985195R Medline TA: J Pharm Sci Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1293-8 Citation Subset: IM |
Affiliation:
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Department of Chemical Engineering, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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12-Hydroxy-5,8,10,14-eicosatetraenoic Acid
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metabolism* Animals Cattle Elasticity Lipids / chemistry Protein Binding Serum Albumin, Bovine / metabolism Surface Properties Surface Tension |
| Chemical | |
Reg. No./Substance:
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0/Lipids; 0/Serum Albumin, Bovine; 59985-28-3/12-Hydroxy-5,8,10,14-eicosatetraenoic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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