Document Detail


Study of amyloids using yeast.
MedLine Citation:
PMID:  22528100     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We detail some of the genetic, biochemical, and physical methods useful in studying amyloids in yeast, particularly the yeast prions. These methods include cytoduction (cytoplasmic mixing), infection of cells with prion amyloids, use of green fluorescent protein fusions with amyloid-forming proteins for cytology, protein purification and amyloid formation, and electron microscopy of filaments.
Authors:
Reed B Wickner; Dmitry Kryndushkin; Frank Shewmaker; Ryan McGlinchey; Herman K Edskes
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Intramural    
Journal Detail:
Title:  Methods in molecular biology (Clifton, N.J.)     Volume:  849     ISSN:  1940-6029     ISO Abbreviation:  Methods Mol. Biol.     Publication Date:  2012  
Date Detail:
Created Date:  2012-04-24     Completed Date:  2012-08-08     Revised Date:  2013-06-25    
Medline Journal Info:
Nlm Unique ID:  9214969     Medline TA:  Methods Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  321-46     Citation Subset:  IM    
Affiliation:
Laboratory of Biochemistry and Genetics, National Institute of Diabetes Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD, USA. wickner@helix.nih.gov
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MeSH Terms
Descriptor/Qualifier:
Amyloid / chemistry*,  genetics,  isolation & purification,  metabolism*
Aspartic Acid / analogs & derivatives,  metabolism
Biological Transport
Chromatography, Liquid
Electrophoresis, Polyacrylamide Gel
Endopeptidase K / metabolism
Gene Fusion
Genetic Vectors / genetics
Luminescent Proteins / genetics,  metabolism
Magnetic Resonance Spectroscopy
Mass Spectrometry
Microscopy, Electron, Transmission
Phenotype
Prions / chemistry,  genetics,  isolation & purification,  metabolism
Protein Multimerization
Protein Structure, Secondary
Protein Structure, Tertiary
Proteolysis
Saccharomyces cerevisiae / cytology,  genetics,  metabolism*
Saccharomyces cerevisiae Proteins / chemistry*,  genetics,  isolation & purification,  metabolism*
Transformation, Genetic
Uracil / metabolism
Grant Support
ID/Acronym/Agency:
ZIA DK024950-04/DK/NIDDK NIH HHS; ZIA DK024950-05/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Amyloid; 0/Luminescent Proteins; 0/Prions; 0/Saccharomyces cerevisiae Proteins; 56-84-8/Aspartic Acid; 66-22-8/Uracil; 923-37-5/ureidosuccinic acid; EC 3.4.21.64/Endopeptidase K
Comments/Corrections

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