Document Detail


Study of alpha-lipoic acid penetration in the human aqueous after topical administration.
MedLine Citation:
PMID:  20456443     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: To determine the concentration of alpha-lipoic acid in the aqueous and investigate if its topical instillation can increase quantities. METHODS: A total of 70 patients selected to undergo cataract surgery were randomly divided into two groups. Group 1 was used as a control group; for the patients in Group 2, a single instillation of alpha-lipoic acid eye drops (1%) was administered. Immediately before surgery, an aliquot of 40-120 microL of aqueous was aspirated. The individual aspirations were combined to constitute pools representing time intervals with respect to administration. The levels of alpha-lipoic acid in the aqueous were measured using gas chromatography/mass spectrometry. RESULTS: Pool 0 was created by combining the samples of aqueous obtained from the patients in Group 1, the control group; and the level of alpha-lipoic acid was 27.5 + 2.6 ng/mL. In the other pools, the time interval between the administration of the eye drops and sampling was, respectively, 23 min, 53 min, 72 min, 93 min and 114 min; and the level of alpha-lipoic acid was 33.0 + 10.8 ng/mL, 52.0 + 2.5 ng/mL, 86.7 + 2.5 ng/mL, 91.2 + 2.5 ng/mL and 80.3 + 2.5 ng/mL. CONCLUSION: Our study demonstrates the presence of alpha-lipoic acid in the aqueous and indicates that its concentration increases after it is administered in the form of eye drops, reaching maximum values after around 93 min. The concentrations that are achieved in the anterior chamber allow us to theorize the possibility of exploiting the antioxidant properties of alpha-lipoic acid.
Authors:
Carlo Cagini; Arianna Leontiadis; Maria A Ricci; Anna Bartolini; Annalisa Dragoni; Roberto M Pellegrino
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Publication Detail:
Type:  Journal Article; Randomized Controlled Trial; Validation Studies     Date:  2010-04-29
Journal Detail:
Title:  Clinical & experimental ophthalmology     Volume:  38     ISSN:  1442-9071     ISO Abbreviation:  Clin. Experiment. Ophthalmol.     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-08-13     Completed Date:  2010-10-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100896531     Medline TA:  Clin Experiment Ophthalmol     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  572-6     Citation Subset:  IM    
Affiliation:
Department of Ophthalmology, University of Perugia, Perugia, Italy. carlocagini@hotmail.com
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MeSH Terms
Descriptor/Qualifier:
Administration, Topical
Aged
Aged, 80 and over
Antioxidants / administration & dosage,  pharmacokinetics*
Aqueous Humor / metabolism*
Calibration
Cornea / metabolism
Female
Gas Chromatography-Mass Spectrometry
Humans
Male
Middle Aged
Ophthalmic Solutions / administration & dosage,  pharmacokinetics*
Thioctic Acid / administration & dosage,  pharmacokinetics*
Chemical
Reg. No./Substance:
0/Antioxidants; 0/Ophthalmic Solutions; 62-46-4/Thioctic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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