Document Detail

Study of aflatoxin B1 and ochratoxin A production by natural microflora and Aspergillus parasiticus in black and green olives of Greek origin.
MedLine Citation:
PMID:  16943059     Owner:  NLM     Status:  MEDLINE    
Aflatoxin B1 (AFB1) is a carcinogenic metabolite produced by certain Aspergillus species. Ochratoxin A (OTA) is classified as "possible carcinogen" and it is a metabolite of Aspergillus ochraceus and Penicillium verrucosum. Fungi contaminate natural and processed olives which support AFB1 and OTA production. The aim of this study was to compare and investigate AFB1 and OTA production in three different varieties of damaged olives. For each variety two different treatments were applied: (1) olives with natural microflora and (2) olives inoculated with A. parasiticus after natural microflora elimination. AFB1 and OTA have been extracted simultaneously from olives, purified with immunoaffinity columns and quantitated by HPLC using fluorescence detector. The recoveries and detection limits of AFB1 and OTA were 94% and 0.15 ng AFB1 g(-1) and 102.7%, 0.41 ng OTA g(-1) respectively. Results showed that, meanwhile OTA was not found in any olive sample, AFB(1) production within the three varieties of olives with natural microflora was significantly (P< or =0.05) different regarding their substrate and time of incubation (18 days). AFB1 production in two different varieties of black olives after inoculation by A. parasiticus was not significantly higher compared with control samples. On the contrary, AFB1 production in green olives was stimulated after the 12th day. Additionally, investigation on the occurrence of AFB1 and OTA in 30 samples of olives and olive pasta from Athens market showed OTA's presence in two samples of olives contaminated at the levels of 1.18 and 1.86 ng OTA g(-1). Aflatoxin B1 was found at levels 0.15-1.13 ng AFB1 g(-1) in all samples tested.
Stavroula Ghitakou; Kostas Koutras; Eleni Kanellou; Panagiota Markaki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-03-23
Journal Detail:
Title:  Food microbiology     Volume:  23     ISSN:  0740-0020     ISO Abbreviation:  Food Microbiol.     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-08-31     Completed Date:  2006-09-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8601127     Medline TA:  Food Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  612-21     Citation Subset:  IM    
Department of Food Chemistry, School of Chemistry, University of Athens, Panepistimiopolis Zografou, GR 157 84 Athens, Greece.
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MeSH Terms
Aflatoxin B1 / analysis*,  biosynthesis
Aspergillus / metabolism*
Carcinogens / analysis
Chromatography, High Pressure Liquid
Food Contamination / analysis*
Food Microbiology
Ochratoxins / analysis*,  biosynthesis
Olea / chemistry*,  microbiology
Penicillium / metabolism
Reg. No./Substance:
0/Carcinogens; 0/Ochratoxins; 1162-65-8/Aflatoxin B1; 303-47-9/ochratoxin A

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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