Document Detail


Study of acute chagasic mice under immunosuppressive therapy by cyclosporin A : modulation and confocal analysis of inflammatory reaction.
MedLine Citation:
PMID:  10708805     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The in vivo effects of cyclosporin A (CsA) on Trypanosoma cruzi infection were examined using different schedules of the drug in mice infected with the Y strain. Parasitaemia at day 8 after infection among CsA-treated animals was usually higher than control infected non-treated mice. On the other hand, mortality analysis showed that animals CsA-treated either with 200 mg/kg 2 days before infection or with therapeutic doses (10 mg/kg every other day) showed almost the same mean time of death (35.8 and 38.2 days, respectively). In these groups mice died 50% less than control infected non-treated ones. The mean time of death in the animals treated with 200 mg/kg 5 days after infection and in infected non-treated control mice were respectively 29.0 and 22.6 days. The kinetics analysis of the leukocyte population of animals treated with a single dose of 200 mg/kg of CsA before or after infection did not show the alternate pattern of leukopenia/leukocytosis observed in control groups of infected mice but differential cell counts indicated a modulatory action upon circulating leukocytes of therapeutic doses of CsA. The animals treated with any of the CsA schedules showed a moderate to intense diffuse inflammatory reaction exhibiting mainly mononuclear cells in the heart. Immunofluorescence analysis by confocal microscopy revealed that macrophages are a major component of the inflammatory infiltrate in all groups of CsA-treated mice and also in the control group.
Authors:
K S Calabrese; A S Paradela; T Zaverucha do Valle; R C Tedesco; S Silva; R A Mortara; S C Gonçalves da Costa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Immunopharmacology     Volume:  47     ISSN:  0162-3109     ISO Abbreviation:  Immunopharmacology     Publication Date:  2000 Apr 
Date Detail:
Created Date:  2000-05-10     Completed Date:  2000-05-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7902474     Medline TA:  Immunopharmacology     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  1-11     Citation Subset:  IM; X    
Affiliation:
Laborat orio de Imunomodula-c-ao, Departamento de Protozoologia, Instituto Oswaldo Cruz, Av. Brasil, 4365, Manguinhos, 21045-900, Rio de Janeiro, Brazil. calabres@gene.dbbm.fiocruz.br
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MeSH Terms
Descriptor/Qualifier:
Acute Disease
Animals
CD4-Positive T-Lymphocytes / drug effects,  physiology
CD8-Positive T-Lymphocytes / drug effects,  physiology
Cell Movement
Chagas Disease / drug therapy*,  mortality
Cyclosporine / pharmacology*,  therapeutic use
Female
Immunohistochemistry
Immunosuppressive Agents / pharmacology,  therapeutic use
Kinetics
Leukocyte Count / drug effects
Lymphocyte Count / drug effects
Macrophages / drug effects,  physiology
Mice
Microscopy, Confocal
Monocytes / cytology,  drug effects
Myocarditis / drug therapy,  parasitology
Myocardium / pathology
Organ Size / drug effects
Parasitemia / drug therapy
Spleen / pathology
Thymus Gland / pathology
Time Factors
Trypanosomiasis / drug therapy,  mortality
Chemical
Reg. No./Substance:
0/Immunosuppressive Agents; 59865-13-3/Cyclosporine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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